Identification of the Minimal Glycotope of Streptococcus pneumoniae 7F Capsular Polysaccharide using Synthetic Oligosaccharides

被引：16

作者：

Menova, Petra ^{[1
,3
]}

Sella, Mauro ^{[1
]}

Sellrie, Katrin ^{[1
]}

Pereira, Claney L. ^{[1
,4
]}

Seeberger, Peter H. ^{[1
,2
]}

机构：

[1] Max Planck Inst Colloids & Interphases, Dept Biomol Syst, Muhlenberg 1, D-14476 Potsdam, Germany

[2] Free Univ Berlin, Dept Chem & Biochem, Arnimallee 22, D-14195 Berlin, Germany

[3] Univ Chem & Technol, Tech 5, Prague 16628, Czech Republic

[4] Vaxxilon Deutschland GmbH, Magnusstr 11, D-12489 Berlin, Germany

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2018年 / 24卷 / 16期

关键词：

glycan arrays; minimal glycotopes; serotype; 7F; Streptococcus pneumoniae; total synthesis; TYPE-7F;

D O I：

10.1002/chem.201705379

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Streptococcus pneumoniae causes life-threatening diseases including meningitis, pneumonia and sepsis. Existing glycoconjugate vaccines based on purified capsular polysaccharides are widely used and help to prevent millions of deaths every year. Herein, the total syntheses of oligosaccharides resembling portions of the S. pneumoniae serotype 7F (ST7F) capsular polysaccharide repeating unit are report-ed. To define minimal glycan epitopes, glycan microarrays containing the synthetic oligosaccharides were used to screen human reference serum and revealed that both side chains of the ST7F play a key role in antigen recognition. The identification of protective minimal epitopes is vital to design efficient semi-and fully-synthetic glycoconjugate vaccines.

引用

收藏

页码：4181 / 4187

页数：7

相关论文

共 15 条

[1] STRUCTURAL STUDIES OF THE CAPSULAR POLYSACCHARIDE FROM STREPTOCOCCUS-PNEUMONIAE TYPE-7A [J].

BACKMANMARKLUND, I ;

JANSSON, PE ;

LINDBERG, B ;

HENRICHSEN, J .

CARBOHYDRATE RESEARCH, 1990, 198 (01) :67-77

[2] Trichloroisocyanuric acid (TCCA): an efficient green reagent for activation of thioglycosides toward hydrolysis [J].

Basu, Nabamita ;

Maity, Sajal Kumar ;

Chaudhury, Aritra ;

Ghosh, Rina .

CARBOHYDRATE RESEARCH, 2013, 369 :10-13

[3] The 13-valent pneumococcal conjugate vaccine (PCV13) elicits cross-functional opsonophagocytic killing responses in humans to Streptococcus pneumoniae serotypes 6C and 7A [J].

Cooper, David ;

Yu, Xinhong ;

Sidhu, Mohinder ;

Nahm, Moon H. ;

Fernsten, Philip ;

Jansen, Kathrin U. .

VACCINE, 2011, 29 (41) :7207-7211

[4] The rise and fall of pneumococcal serotypes carried in the PCV era [J].

Devine, Vanessa T. ;

Cleary, David W. ;

Jefferies, Johanna M. C. ;

Anderson, Rebecca ;

Morris, Denise E. ;

Tuck, Andrew C. ;

Gladstone, Rebecca A. ;

O'Doherty, Grace ;

Kuruparan, Priyasharmila ;

Bentley, Stephen D. ;

Faust, Saul N. ;

Clarke, Stuart C. .

VACCINE, 2017, 35 (09) :1293-1298

[5]

ECDC, 2016, ANN EP REP

[6] A Streptococcus pneumoniae Type 2 Oligosaccharide Glycoconjugate Elicits Opsonic Antibodies and Is Protective in an Animal Model of Invasive Pneumococcal Disease [J].

Emmadi, Madhu ;

Khan, Naeem ;

Lykke, Lennart ;

Reppe, Katrin ;

Parameswarappa, Sharavathi G. ;

Lisboa, Marilda P. ;

Wienhold, Sandra-Maria ;

Witzenrath, Martin ;

Pereira, Claney L. ;

Seeberger, Peter H. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2017, 139 (41) :14783-14791

[7] Deciphering Antigenic Determinants of Streptococcus pneumoniae Serotype 4 Capsular Polysaccharide using Synthetic Oligosaccharides [J].

Geissner, Andreas ;

Pereira, Claney L. ;

Leddermann, Melanie ;

Anish, Chakkumkal ;

Seeberger, Peter H. .

ACS CHEMICAL BIOLOGY, 2016, 11 (02) :335-344

[8] Pneumococcal Capsules and Their Types: Past, Present, and Future [J].

Geno, K. Aaron ;

Gilbert, Gwendolyn L. ;

Song, Joon Young ;

Skovsted, Ian C. ;

Klugman, Keith P. ;

Jones, Christopher ;

Konradsen, Helle B. ;

Nahm, Moon H. .

CLINICAL MICROBIOLOGY REVIEWS, 2015, 28 (03) :871-899

[9] Establishment of a New Human Pneumococcal Standard Reference Serum, 007sp [J].

Goldblatt, D. ;

Plikaytis, B. D. ;

Akkoyunlu, M. ;

Antonello, J. ;

Ashton, L. ;

Blake, M. ;

Burton, R. ;

Care, R. ;

Durant, N. ;

Feavers, I. ;

Fernsten, P. ;

Fievet, F. ;

Giardina, P. ;

Jansen, K. ;

Katz, L. ;

Kierstead, L. ;

Lee, L. ;

Lin, J. ;

Maisonneuve, J. ;

Nahm, M. H. ;

Raab, J. ;

Romero-Steiner, S. ;

Rose, C. ;

Schmidt, D. ;

Stapleton, J. ;

Carlone, G. M. .

CLINICAL AND VACCINE IMMUNOLOGY, 2011, 18 (10) :1728-1736

[10] APPLICATION OF HIGH-RESOLUTION NMR-SPECTROSCOPY TO THE ELUCIDATION OF THE STRUCTURE OF THE SPECIFIC CAPSULAR POLYSACCHARIDE OF STREPTOCOCCUS-PNEUMONIAE TYPE-7F [J].

MOREAU, M ;

RICHARDS, JC ;

PERRY, MB ;

KNISKERN, PJ .

CARBOHYDRATE RESEARCH, 1988, 182 (01) :79-99