Potentiometric Cholesterol Biosensor Based on ZnO Nanowalls and Stabilized Polymerized Lipid Film

被引：40

作者：

Psychoyios, Vasillios N. ^{[1
]}

Nikoleli, Georgia-Paraskevi ^{[1
]}

Tzamtzis, Nikolaos ^{[1
]}

Nikolelis, Dimitrios P. ^{[2
]}

Psaroudakis, Nikolas ^{[3
]}

Danielsson, Bengt ^{[4
]}

Israr, Muhammad Qadir ^{[5
]}

Willander, Magnus ^{[5
]}

机构：

[1] Natl Tech Univ Athens, Lab Inorgan & Analyt Chem, Sch Chem Engn, Dept 1, Athens 15780, Greece

[2] Univ Athens, Dept Chem, Environm Chem Lab, G-15771 Athens, Greece

[3] Univ Athens, Dept Chem, Inorgan Chem Lab, GR-15771 Athens, Greece

[4] Acromed Invest AB, S-22643 Lund, Sweden

[5] Linkoping Univ, Dept Sci & Technol, SE-60174 Norrkoping, Sweden

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 02期

关键词：

ZnO nanowalls; Potentiometric nanosensors; Cholesterol oxidase; Cholesterol; Stabilized lipid films; OXIDASE; NANORODS; ESTERASE; MEMBRANE;

D O I：

10.1002/elan.201200591

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel potentiometric cholesterol biosensor was fabricated by immobilization of cholesterol oxidase into stabilized lipid films using zinc oxide (ZnO) nanowalls as measuring electrode. Cholesterol oxidase was incorporated into the lipid film prior polymerization on the surface of ZnO nanowalls resulting in a sensitive, selective, stable and reproducible cholesterol biosensor. The potentiometric response was 57mV/ decade concentration. The sensor response had no interferences by normal concentrations of ascorbic acid, glucose, and urea, proteins and lipids. The present biosensor could be implanted in the human body because of the biocompatibility of the lipid film.

引用

页码：367 / 372

页数：6

共 30 条

[11] ZnO nanotube based dye-sensitized solar cells [J].

Martinson, Alex B. F. ;

Elam, Jeffrey W. ;

Hupp, Joseph T. ;

Pellin, Michael J. .

NANO LETTERS, 2007, 7 (08) :2183-2187

[12] Role of cholesterol and lipid organization in disease [J].

Maxfield, FR ;

Tabas, I .

NATURE, 2005, 438 (7068) :612-621

[13] Controlled synthesis of ZnO from nanospheres to micro-rods and its gas sensing studies [J].

Navale, Shalaka C. ;

Gosavi, S. W. ;

Mulla, I. S. .

TALANTA, 2008, 75 (05) :1315-1319

[14] Sonochemical approach for rapid growth of zinc oxide nanowalls [J].

Nayak, Avinash P. ;

Katzenmeyer, Aaron M. ;

Gosho, Yasuhiro ;

Tekin, Bayram ;

Islam, M. Saif .

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 2012, 107 (03) :661-667

[15] Optical portable biosensors based on stabilized lipid membrane for the rapid detection of doping materials in human urine [J].

Nikolelis, Dimitrios P. ;

Raftopoulou, Garyfallia ;

Chatzigeorgiou, Petros ;

Nikoleli, Georgia-Paraskevi ;

Viras, Kyriakos .

SENSORS AND ACTUATORS B-CHEMICAL, 2008, 130 (02) :577-582

[16] Stabilized lipid membrane based biosensors with incorporated enzyme for repetitive uses [J].

Nikolelis, Dimitrios P. ;

Raftopoulou, Garyfallia ;

Nikoleli, Georgia-Paraskevi ;

Simantiraki, Maria .

ELECTROANALYSIS, 2006, 18 (24) :2467-2474

[17] Stabilized Lipid Films in Electrochemical Biosensors [J].

Nikolelis, Dimitrios P. ;

Hianik, Tibor ;

Nikoleli, Georgia-Paraskevi .

ELECTROANALYSIS, 2010, 22 (23) :2747-2763

[18] BILAYER-LIPID MEMBRANES FOR FLOW-INJECTION MONITORING OF ACETYLCHOLINE, UREA, AND PENICILLIN [J].

NIKOLELIS, DP ;

SIONTOROU, CG .

ANALYTICAL CHEMISTRY, 1995, 67 (05) :936-944

[19] Flow injection analysis of carbofuran in foods using air stable lipid film based acetylcholinesterase biosensor [J].

Nikolelis, DP ;

Simantiraki, MG ;

Siontorou, CG ;

Toth, K .

ANALYTICA CHIMICA ACTA, 2005, 537 (1-2) :169-177

[20] An optical spot test for the detection of dopamine in human urine using stabilized in air lipid films [J].

Nikolelis, DP ;

Drivelos, DA ;

Simantiraki, MG ;

Koinis, S .

ANALYTICAL CHEMISTRY, 2004, 76 (08) :2174-2180

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