Potentiometric Cholesterol Biosensor Based on ZnO Nanowalls and Stabilized Polymerized Lipid Film

被引：40

作者：

Psychoyios, Vasillios N. ^{[1
]}

Nikoleli, Georgia-Paraskevi ^{[1
]}

Tzamtzis, Nikolaos ^{[1
]}

Nikolelis, Dimitrios P. ^{[2
]}

Psaroudakis, Nikolas ^{[3
]}

Danielsson, Bengt ^{[4
]}

Israr, Muhammad Qadir ^{[5
]}

Willander, Magnus ^{[5
]}

机构：

[1] Natl Tech Univ Athens, Lab Inorgan & Analyt Chem, Sch Chem Engn, Dept 1, Athens 15780, Greece

[2] Univ Athens, Dept Chem, Environm Chem Lab, G-15771 Athens, Greece

[3] Univ Athens, Dept Chem, Inorgan Chem Lab, GR-15771 Athens, Greece

[4] Acromed Invest AB, S-22643 Lund, Sweden

[5] Linkoping Univ, Dept Sci & Technol, SE-60174 Norrkoping, Sweden

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 02期

关键词：

ZnO nanowalls; Potentiometric nanosensors; Cholesterol oxidase; Cholesterol; Stabilized lipid films; OXIDASE; NANORODS; ESTERASE; MEMBRANE;

D O I：

10.1002/elan.201200591

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel potentiometric cholesterol biosensor was fabricated by immobilization of cholesterol oxidase into stabilized lipid films using zinc oxide (ZnO) nanowalls as measuring electrode. Cholesterol oxidase was incorporated into the lipid film prior polymerization on the surface of ZnO nanowalls resulting in a sensitive, selective, stable and reproducible cholesterol biosensor. The potentiometric response was 57mV/ decade concentration. The sensor response had no interferences by normal concentrations of ascorbic acid, glucose, and urea, proteins and lipids. The present biosensor could be implanted in the human body because of the biocompatibility of the lipid film.

引用

页码：367 / 372

页数：6

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