Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm Helicoverpa armigera

被引：13

作者：

Jin, Minghui ^{[1
,2
]}

Liao, Chongyu ^{[1
]}

Chakrabarty, Swapan ^{[1
]}

Wu, Kongming ^{[2
]}

Xiao, Yutao ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China

[2] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, West Yuanmingyuan Rd, Beijing 100193, Peoples R China

来源：

TOXINS | 2019年 / 11卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Helicoverpa armigera; Bt; midgut; transcriptome; peritrophic matrix; proteome; BACILLUS-THURINGIENSIS-RESISTANT; LEPIDOPTERA-NOCTUIDAE; INSECT RESISTANCE; INSTAR LARVAE; MEMBRANE PM; TOXINS; GUT; DIVERSITY; MIDGUT; PROTEINASES;

D O I：

10.3390/toxins11020092

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Crystalline (Cry) proteins from Bacillus thuringiensis (Bt) are widely used in sprays and transgenic crops to control insect pests, but the evolution of insect resistance threatens their long-term use. Different resistance mechanisms have been identified, but some have not been completely elucidated. Here, the transcriptome of the midgut and proteome of the peritrophic matrix (PM) were comparatively analyzed to identify potential mechanism of resistance to Cry1Ac in laboratory-selected strain XJ10 of Helicoverpa armigera. This strain had a 146-fold resistance to Cry1Ac protoxin and 45-fold resistance to Cry1Ac activated toxin compared with XJ strain. The mRNA and protein levels for several trypsin genes were downregulated in XJ10 compared to the susceptible strain XJ. Furthermore, 215 proteins of the PM were identified, and nearly all had corresponding mRNAs in the midgut. These results provide new insights that the PM may participate in Bt resistance.

引用

页数：14

共 50 条

[21] Cis-mediated down-regulation of a trypsin gene associated with Bt resistance in cotton bollworm [J].

Liu, Chenxi ;

Xiao, Yutao ;

Li, Xianchun ;

Oppert, Brenda ;

Tabashnik, Bruce E. ;

Wu, Kongming .

SCIENTIFIC REPORTS, 2014, 4

[22] Binding of three Cry1A toxins in resistant and susceptible strains of cotton bollworm (Helicoverpa armigera) [J].

Luo, Shudong ;

Wang, Guirong ;

Liang, Gemei ;

Wu, Kong Ming ;

Bai, Lianyang ;

Ren, Xinguo ;

Guo, Yuyuan .

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2006, 85 (02) :104-109

[23] serpentine and vermiform encode matrix proteins with chitin binding and deacetylation domains that limit tracheal tube length in Drosophila [J].

Luschnig, S ;

Bätz, T ;

Armbruster, K ;

Krasnow, MA .

CURRENT BIOLOGY, 2006, 16 (02) :186-194

[24] Are Bt crops safe? [J].

Mendelsohn, M ;

Kough, J ;

Vaituzis, Z ;

Matthews, K .

NATURE BIOTECHNOLOGY, 2003, 21 (09) :1003-1009

[25] Proteinase-mediated insect resistance to Bacillus thuringiensis toxins [J].

Oppert, B ;

Kramer, KJ ;

Beeman, RW ;

Johnson, D ;

McGaughey, WH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (38) :23473-23476

[26] Bacillus thuringiensis insecticidal three-domain Cry toxins: mode of action, insect resistance and consequences for crop protection [J].

Pardo-Lopez, Liliana ;

Soberon, Mario ;

Bravo, Alejandra .

FEMS MICROBIOLOGY REVIEWS, 2013, 37 (01) :3-22

[27] Insect feeding mobilizes a unique plant defense protease that disrupts the peritrophic matrix of caterpillars [J].

Pechan, T ;

Cohen, A ;

Williams, WP ;

Luthe, DS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (20) :13319-13323

[28] Resistance of Helicoverpa armigera to Cry1Ac toxin from Bacillus thuringiensis is due to improper processing of the protoxin [J].

Rajagopal, Raman ;

Arora, Naresh ;

Sivakumar, Swaminathan ;

Rao, Nagarjun G. V. ;

Nimbalkar, Sharad A. ;

Bhatnagar, Raj K. .

BIOCHEMICAL JOURNAL, 2009, 419 :309-316

[29] Bacillus thuringiensis and its pesticidal crystal proteins [J].

Schnepf, E ;

Crickmore, N ;

Van Rie, J ;

Lereclus, D ;

Baum, J ;

Feitelson, J ;

Zeigler, DR ;

Dean, DH .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) :775-+

[30] Characterization of a novel gut-specific chitinase gene from the human malaria vector Anopheles gambiae [J].

Shen, ZC ;

JacobsLorena, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (46) :28895-28900

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