Integrin β1 mediates epithelial growth factor-induced invasion in human ovarian cancer cells

被引：28
作者：
Lau, Man-Tat [1 ]
So, Wai-Kin [1 ]
Leung, Peter C. K. [1 ]
机构：
[1] Univ British Columbia, Dept Obstet & Gynecol, Child & Family Res Inst, Vancouver, BC V6H 3N1, Canada
来源：
CANCER LETTERS | 2012年 / 320卷 / 02期
基金：
加拿大健康研究院;
关键词：
EGF; ITGB1; MAPK/ERK; Ovarian cancer; EXTRACELLULAR-MATRIX; RECEPTOR EXPRESSION; BETA-1-INTEGRIN; ADHESION; MIGRATION; PATHWAY; FAMILY; SNAIL; CD44; MAPK;
D O I：
10.1016/j.canlet.2012.02.028
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Integrins function as cell-extracellular matrix adhesion proteins and have been implicated in tumor progression. In ovarian tumors, elevated integrin beta 1 expression correlates with high clinical stage and poor patient survival. In this study, we report that EGF treatment up-regulated integrin beta 1 mRNA and protein levels in ovarian cancer cells. Moreover, pharmacological inhibition of MEK totally abolished EGF-induced integrin beta 1 up-regulation and cell invasion suggesting that MAPK/ERK signaling is required for EGF-induced integrin beta 1 up-regulation and cell invasion. Furthermore, we found that knockdown of integrin beta 1 expression reduced the intrinsic invasiveness of ovarian cancer cells and the EGF-induced cell invasion. Finally, we found that overexpression of integrin beta 1 was sufficient to promote ovarian cancer cell invasion. This study demonstrates that integrin beta 1 mediates EGF-induced cell invasion in ovarian cancer. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：198 / 204
页数：7
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