The identification of a sex-specific DNA marker in the ostrich using a random amplified polymorphic DNA (RAPD) assay

被引：30
|
作者：
Bello, N
Sánchez, A [1 ]
机构：
[1] Univ Autonoma Barcelona, Fac Vet, Dept Patol & Prod Anim, Unitat Genet & Millora, Bellaterra 08193, Spain
[2] ES Genet Estruc, GEN, Figueres 17600, Spain
来源：
MOLECULAR ECOLOGY | 1999年 / 8卷 / 04期
关键词：
ostrich; PCR; RAPD; ratites; SCAR; sex-marker;
D O I：
10.1046/j.1365-294x.1999.00549.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
PCR-based techniques described to date for sex diagnosis in birds are not useful in ratites. We report here the identification of a W-linked marker in the ostrich (Struthio camelus) which allows gender diagnosis in chicks or juvenile birds. DNA from 10 females and 11 males was used to prepare two pools for each sex. Two-hundred different l0-mer primers of arbitrary sequence were used to screen those pools using a random amplified polymorphic DNA (RAPD) assay. One primer (D 10) generated a female-specific band. Sex specificity was confirmed by testing the 21 animals individually. The candidate DNA fragment was cloned and sequenced. Longer primers were designed to optimize a sex-specific PCR which will be useful in diagnosis.
引用
收藏
页码：667 / 669
页数：3
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