In Situ Monitoring of Bindings between Dasatinib and Its Target Protein Kinases Using Magnetic Nanoparticles in Live Cells

被引：10

作者：

Kim, Dae-Joong ^{[1
]}

Yi, Yong-Weon ^{[1
]}

Kim, Jin Hwan ^{[1
]}

机构：

[1] CGK Co Ltd, Daejeon Bioventure Town, Taejon 305811, South Korea

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 49期

关键词：

D O I：

10.1021/ja8063843

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a novel technology, called InCell IT, for in situ monitoring of bindings between a small molecule kinase inhibitor and its target protein kinases in live cells using a kinase inhibitor, dasatinib, as a model compound. Streptavidin-attached MNPs were coated by biotinylated dasatinib, and then these dasatinib-MNPs were transferred into cells. In the cells, the MNPs were aligned to the same direction of the magnetic field and EGFP-tagged target protein kinases were bound to dasatinib-MNPs. Using this technology, we demonstrated the bindings between dasatinib and its target protein kinases including SRC, ABL1, and CSK in live cells. The specificity of these bindings was also confirmed by cold competition using unbiotinylated dasatinib in the same cells.

引用

页码：16466 / +

页数：3

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