cDNA cloning and characterization of Type I procollagen α1 chain in the skate Raja kenojei

被引：5

作者：

Hwang, JH ^{[1
]}

Yokoyama, Y ^{[1
]}

Mizuta, S ^{[1
]}

Yoshinaka, R ^{[1
]}

机构：

[1] Fukui Prefectural Univ, Fac Biotechnol, Dept Marine Biosci, Fukui 9170003, Japan

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2006年 / 144卷 / 01期

关键词：

cDNA cloning; collagen; elasmobranch; embryo; fish; nucleotide sequence; RT-PCR; skate;

D O I：

10.1016/j.cbpb.2005.12.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length cDNA of the Type I procollagen alpha 1 [pro-alpha 1(I)] chain (4388 bp), coding for 1463 amino acid residues in the total length, was determined by RACE PCR using a cDNA library constructed from 4-week embryo of the skate Raja kenojei. The helical region of the skate pro alpha 1(I) chain consisted of 1014 amino acid residues - the same as other fibrillar collagen a chains from higher vertebrates. Comparison on denaturation temperatures of Type I collagens from the skate, rainbow trout (Oncorynchus mykiss) and rat (Rattus norvegicus) revealed that the number of Gly-Pro-Pro and Gly-Gly in the alpha 1(I) chains could be directly related to the thermal stability of the helix. The expression property of the skatepro-alpha(I) chain mRNA and phylogenetic analysis with other vertebrate pro-alpha 1(I) chains suggested that skate pro-alpha 1(I) chain could be a precursor form of the skate Type I collagen alpha I chain. The present study is the first evidence for the primary structure of full-length pro-alpha 1(I) chain in an elasmobranch. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1 / 10

页数：10

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