In this work we used natural and modified cyclodextrins (beta-CD and HP-beta-CD) as encapsulating agents to improve citronellal properties. Using fluorimetric techniques, its aggregation behavior was studied for the first time. Its critical micellar concentration was seen to vary with the presence of cyclodextrins, which form 1:1 stoichiometry complexes with citronellal. The encapsulation constants and the scores obtained by Molecular Docking were correlated. Chromatographic (GC MS) and sensory analysis confirmed that cyclodextrins improve the persistence of the aroma. Finally, the antimicrobial effect of citronellal against Escherichia coli and Bacillus subtilis in the presence and absence of cyclodextrins was studied. A combinatorial effect of citronellal, HP-P-cyclodextrin and Glucobay (R) as an antimicrobial mixture was observed. The results of this study not only demonstrated the potential of CD mixtures, but also that the growth caused by CD digestion may sometimes be greater that the antimicrobial effect of the agents used in this study.
机构:
Univ Lisbon, Fac Farm, I Med UL, Res Inst Med & Pharmaceut Sci, P-1649003 Lisbon, PortugalUniv Lisbon, Fac Farm, I Med UL, Res Inst Med & Pharmaceut Sci, P-1649003 Lisbon, Portugal
机构:
Univ Porto, Fac Engn, Dept Chem Engn, LEPABE, P-4200465 Oporto, Portugal
Univ Porto, Fac Sci, Dept Chem & Biochem, CIQUP, P-4169007 Oporto, Portugal
Univ Tras Os Montes & Alto Douro, Dept Vet Med, Vet & Anim Sci Res Ctr CECAV, P-5000801 Vila Real, PortugalRes Ctr Food & Dev, CIAD AC, Hermosillo 83000, Sonora, Mexico
机构:
Univ Lisbon, Fac Farm, I Med UL, Res Inst Med & Pharmaceut Sci, P-1649003 Lisbon, PortugalUniv Lisbon, Fac Farm, I Med UL, Res Inst Med & Pharmaceut Sci, P-1649003 Lisbon, Portugal
机构:
Univ Porto, Fac Engn, Dept Chem Engn, LEPABE, P-4200465 Oporto, Portugal
Univ Porto, Fac Sci, Dept Chem & Biochem, CIQUP, P-4169007 Oporto, Portugal
Univ Tras Os Montes & Alto Douro, Dept Vet Med, Vet & Anim Sci Res Ctr CECAV, P-5000801 Vila Real, PortugalRes Ctr Food & Dev, CIAD AC, Hermosillo 83000, Sonora, Mexico