Targeting Essential Genes in Salmonella enterica Serovar Typhimurium with Antisense Peptide Nucleic Acid

被引：28
|
作者：
Soofi, Muhammad A. [1 ]
Seleem, Mohamed N. [1 ]
机构：
[1] Purdue Univ, Dept Comparat Pathobiol, Coll Vet Med, W Lafayette, IN 47907 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 12期
关键词：
SINGLE-STRANDED-DNA; ESCHERICHIA-COLI; INHIBITION; GROWTH; EXPRESSION; PNA; TRANSLATION; INFECTION; OLIGOMER; BACTERIA;
D O I：
10.1128/AAC.01437-12
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We investigated the capability of antisense peptide nucleic acids (PNAs) conjugated to the (KFF)(3)K cell-penetrating peptide to target possible essential genes (ligA, rpoA, rpoD, engA, tsf, and kdtA) in Salmonella enterica serovar Typhimurium and inhibit bacterial growth in vitro and in cell culture. All targeted PNA-based gene inhibition has shown great potency in gene expression inhibition in a sequence-specific and dose-dependent manner at micromolar concentrations. Among tested PNAs, the anti-rpoA and -rpoD PNAs showed the greatest potency.
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页码：6407 / 6409
页数：3
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