Pectinatus brassicae sp nov., a Gram-negative, anaerobic bacterium isolated from salty wastewater

A novel Gram-negative, non-spore-forming, strictly anaerobic, heterotrophic bacterium, strain TYT, was isolated from salty pickle wastewater. Cells were rod-shaped with comb-like flagella, slightly curved and very variable in length. Optimal growth occurred at 28 degrees C and pH 6.5. Cells were resistant to up to 50 g NaCl I-1. Strain TYT produced acid from glycerol, sucrose, glucose, fructose and mannitol. The main fermentation products from glucose were acetic and propionic acids. Tests for acid phosphatase and naphthol-AS-Bl-phosphohydrolase activities were positive. The major fatty acids were C-14:0 DMA (18.7 %), C-15:0 (15.4 %), anteiso-C-18:1 (15.2 %), C-11:0 (13.3%) and summed feature 5 (C-17:1 omega 7c and/or C-17:2) (11.0 %). The DNA G+C content was 35.9 mol%. 16S rRNA gene sequence-based phylogenetic analysis indicated that strain TYT represented a novel species of the genus Pectinatus (sequence similarity to other members of the genus ranged from 93.2 to 94.8%). Based on its phenotypic, genotypic and phylogenetic characteristics, strain TYT is proposed to represent a novel species, named Pectinatus brassicae sp. nov. (type strain TYT =JCM 17499(T) =DSM 24661(T)).