Enhancement of the binding ability of a ligand for nucleobase recognition by introducing a methyl group

被引：48
作者：
Dai, Q
Xu, CY
Sato, Y
Yoshimoto, K
Nishizawa, S
Teramae, N [1 ]
机构：
[1] Tohoku Univ, Grad Sch Sci, Dept Chem, Sendai, Miyagi 9808578, Japan
[2] JST, CREST, Aoba Ku, Sendai, Miyagi 9808578, Japan
[3] RIKEN, Inst Phys & Chem Res, Bioengn Lab, Wako, Saitama 3510198, Japan
来源：
ANALYTICAL SCIENCES | 2006年 / 22卷 / 02期
关键词：
D O I：
10.2116/analsci.22.201
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5 degrees C; 6.2 x 10(6) M-1 of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteri dine and guanine opposite the AP site in water.
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收藏
页码：201 / 203
页数：3
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