Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity in Pluripotency and Reprogramming

被引：80

作者：

Ding, Junjun ^{[1
,3
]}

Huang, Xin ^{[1
,3
]}

Shao, Ningyi ^{[4
,5
]}

Zhou, Hongwei ^{[1
,3
]}

Lee, Dung-Fang ^{[1
,3
]}

Faiola, Francesco ^{[1
,3
]}

Fidalgo, Miguel ^{[1
,3
]}

Guallar, Diana ^{[1
,3
]}

Saunders, Arven ^{[1
,2
,3
]}

Shliaha, Pavel V. ^{[6
]}

Wang, Hailong ^{[7
]}

Waghray, Avinash ^{[1
,2
,3
]}

Papatsenko, Dmitri ^{[1
,3
]}

Sanchez-Priego, Carlos ^{[1
,3
]}

Li, Dan ^{[1
,2
,3
]}

Yuan, Ye ^{[1
,2
,3
]}

Lemischka, Ihor R. ^{[1
,2
,3
,8
]}

Shen, Li

Kelley, Kevin ^{[3
]}

Deng, Haiteng ^{[9
]}

Shen, Xiaohua ^{[9
]}

Wang, Jianlong ^{[1
,2
,3
]}

机构：

[1] Icahn Sch Med Mt Sinai, Black Family Stem Cell Inst, New York, NY 10029 USA

[2] Icahn Sch Med Mt Sinai, Grad Sch Biomed Sci, New York, NY 10029 USA

[3] Icahn Sch Med Mt Sinai, Dept Dev & Regenerat Biol, New York, NY 10029 USA

[4] Icahn Sch Med Mt Sinai, Fishberg Dept Neurosci, New York, NY 10029 USA

[5] Icahn Sch Med Mt Sinai, Friedman Brain Inst, New York, NY 10029 USA

[6] Univ Cambridge, Dept Biochem, Cambridge CB2 1QR, England

[7] Xiamen Univ, Organ Transplantat Inst, Xiamen 361102, Fujian Province, Peoples R China

[8] Icahn Sch Med Mt Sinai, Dept Pharmacol & Syst Therapeut, New York, NY 10029 USA

[9] Tsinghua Univ, Sch Med, Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China

来源：

CELL STEM CELL | 2015年 / 16卷 / 06期

关键词：

EMBRYONIC STEM-CELLS; CORE TRANSCRIPTIONAL NETWORK; PROTEIN-INTERACTION NETWORK; SELF-RENEWAL; GENE-EXPRESSION; SOMATIC-CELLS; CHROMATIN; DIFFERENTIATION; COMPLEX; NANOG;

D O I：

10.1016/j.stem.2015.04.001

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage-specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for the core pluripotency factors Oct4, Sox2, and Nanog. In this study, we sought to dissect the molecular control mechanism of SE activity in pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, we identified Tex10 as a key pluripotency factor that plays a functionally significant role in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation at SEs. Tex10 activity is also important for pluripotency and reprogramming in human cells. Our study therefore highlights Tex10 as a core component of the pluripotency network and sheds light on its role in epigenetic control of SE activity for cell fate determination.

引用

页码：653 / 668

页数：16

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