Synthetic Ligand-Coated Magnetic Nanoparticles for Microfluidic Bacterial Separation from Blood

被引：106

作者：

Lee, Jung-Jae ^{[1
,2
]}

Jeong, Kyung Jae ^{[1
,2
]}

Hashimoto, Michinao ^{[1
,2
]}

Kwon, Albert H. ^{[1
,3
]}

Rwei, Alina ^{[1
,2
]}

Shankarappa, Sahadev A. ^{[1
,2
]}

Tsui, Jonathan H. ^{[1
,2
]}

Kohane, Daniel S. ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Lab Biomat & Drug Delivery,Dept Anesthesiol, Div Crit Care Med,Boston Childrens Hosp, Boston, MA 02115 USA

[2] MIT, David H Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

[3] Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

来源：

NANO LETTERS | 2014年 / 14卷 / 01期

关键词：

Sepsis; Zn-DPA; magnetic nanoparticle; microfluidics; bacteria; magnetophoresis; DIRECT ENUMERATION; ANION RECOGNITION; SEVERE SEPSIS; THERAPY; INFECTION; BIOLUMINESCENCE; HEMOFILTRATION; SHOCK; DRUG;

D O I：

10.1021/nl3047305

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Bacterial sepsis is a serious clinical condition that can lead to multiple organ dysfunction and death despite timely treatment with antibiotics and fluid resuscitation. We have developed an approach to clearing bacteria and endotoxin from the bloodstream, using magnetic nanoparticles (MNPs) modified with bis-Zn-DPA, a synthetic ligand that binds to both Gram-positive and Gram-negative bacteria. Magnetic microfluidic devices were used to remove MNPs bound to Escherichia coli, Gram-negative bacterium commonly implicated in bacterial sepsis, from bovine whole blood at flows as high as 60 mL/h, resulting in almost 100% clearance. Such devices could be adapted to clear bacteria from septicemic patients.

引用

页码：1 / 5

页数：5

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