Silicate Fiber-based 3D Cell Culture System for Anticancer Drug Screening

被引：0

作者：

Yamaguchi, Yoshie ^{[1
]}

Deng, Dawei ^{[1
]}

Sato, Yoshinori ^{[1
]}

Hou, Yung-Te ^{[1
]}

Watanabe, Rie ^{[2
]}

Sasaki, Kohei ^{[2
]}

Kawabe, Masaaki ^{[2
]}

Hirano, Eiichi ^{[1
]}

Morinaga, Tetsuo ^{[1
]}

机构：

[1] Japan Bio Prod Co Ltd, Kurume, Fukuoka 8390861, Japan

[2] Japan Vilene Co Ltd, Ibaraki, Japan

来源：

ANTICANCER RESEARCH | 2013年 / 33卷 / 12期

关键词：

Three-dimensional (3D) culture; silicate fiber scaffold; drug resistance; anticancer drug screening; IN-VITRO EVALUATION; COLON-CANCER CELLS; CHEMOTHERAPEUTIC DRUGS; LACTATE-DEHYDROGENASE; SPHEROID MODEL; TUMOR-TISSUE; PENETRATION; RESISTANCE; APOPTOSIS; 3RD-DIMENSION;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Three-dimensional (3D) in vitro cultures can recapitulate the physiological in vivo microenvironment. 3D Modeling techniques have been investigated and applied in anticancer drug screening. Materials and Methods: A silicate fiber scaffold was used for 3D cell cultures, and used to model the efficacy of anticancer drugs, such as mytomicin C and doxorubicin. Results: A unique 3D structure was observed in 13 human tumor cell lines on scaffold, and these cells exhibited higher drug resistance than cells in two-dimensional (2D) cultures. Furthermore, the production of lactate and expression of the nuclear factor-kappa B (NF-kappa B)-regulated genes B cell lymphoma-2 (BCL2), cyclooxygenase-2 (COX2), and vascular endothelial growth factor (VEGF) were higher in 3D cultures than in 2D cultures. Conclusion: These findings suggest that a 3D model using a silicate fiber scaffold can mimic features of cancer, and is also a suitable model for the evaluation of anticancer drugs in vitro.

引用

页码：5301 / 5309

页数：9

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