Stopped-flow fluorimetric determination of amoxycillin and clavulanic acid by partial least-squares multivariate calibration

被引：20
作者：
de la Peña, AM [1 ]
Acedo-Valenzuela, MI [1 ]
Espinosa-Mansilla, A [1 ]
Sánchez-Maqueda, R [1 ]
机构：
[1] Univ Extremadura, Fac Sci, Dept Analyt Chem, E-06071 Badajoz, Spain
来源：
TALANTA | 2002年 / 56卷 / 04期
关键词：
clavulanic acid; amoxycillin; stopped-flow mixing; partial least-squares;
D O I：
10.1016/S0039-9140(01)00612-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Mixtures of amoxycillin and clavulanic acid were determined by using kinetic data in combination with partial least-squares multivariate calibration. The reaction of oxidation of these compounds with cerium(IV) in sulfuric acid medium has been monitored fluori metrically. To follow the kinetics of the reaction, the stopped-flow mixing technique was used. Partial least-squares calibration of the kinetic data allowed the resolution of the analytes investigated in the concentration ranges between 0 and 4 mug ml(-1). The method was applied satisfactorily to several pharmaceutical formulations, including Clavucid, Augmentine, Pangamox, Eupeclanic and Clamoxyl. The results obtained were validated by using an HPLC method. The percentages of recovery range from 91 to 105% for amoxycillin and from 78 to 117% for clavulanic acid, respectively. (C) 2002 Elsevier Science B.V. All rights reserved.
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收藏
页码：635 / 642
页数：8
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