Flow-through immobilized enzyme reactors based on monoliths: I. Preparation of heterogeneous biocatalysts

被引：33

作者：

Vlakh, Evgenia G. ^{[1
,2
]}

Tennikova, Tatiana B. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Inst Macromol Cpds, Bolshoy Pr 31, St Petersburg 199004, Russia

[2] St Petersburg State Univ, Fac Chem, St Petersburg, Russia

来源：

JOURNAL OF SEPARATION SCIENCE | 2013年 / 36卷 / 01期

关键词：

Enzymes; Flow-through systems; Heterogeneous biocatalysts; Immobilized enzyme reactor (IMER); Monoliths; PERFORMANCE MEMBRANE CHROMATOGRAPHY; POROUS POLYMER MONOLITHS; ONLINE PROTEIN DIGESTION; LESS COMMON APPLICATIONS; SOL-GEL; SILICA MONOLITH; AFFINITY-CHROMATOGRAPHY; LIQUID-CHROMATOGRAPHY; TRYPSIN MICROREACTOR; POLY(ETHYLENE GLYCOL);

D O I：

10.1002/jssc.201200594

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of monoliths for realization of solid-phase biocatalytic processes was dramatically extended since the beginning of new century. Different enzyme immobilization techniques regarding these modern stationary phases have been developed, adapted, and optimized within last decade. The choice of enzyme immobilization method depends on material nature and monolith manufacturing. The present review collected, analyzed, and discussed the accessible published data on existing approaches and specialties of preparation of flow-through enzyme reactors based on monoliths.

引用

页码：110 / 127

页数：18

共 12 条

[1] Flow-through immobilized enzyme reactors based on monoliths: II. Kinetics study and application
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