Codominance of Lactobacillus plantarum and obligate heterofermentative lactic acid bacteria during sourdough fermentation

被引:63
作者
Ventimiglia, Giusi [1 ]
Alfonzo, Antonio [1 ]
Galluzzo, Paola [2 ]
Corona, Onofrio [1 ]
Francesca, Nicola [1 ]
Caracappa, Santo [2 ]
Moschetti, Giancarlo [1 ]
Settanni, Luca [1 ]
机构
[1] Univ Palermo, Dipartimento Sci Agr & Forestali, I-90128 Palermo, Italy
[2] Ist Zooprofilatt Sperimentale Sicilia A Mirri, Palermo, Italy
关键词
Bacterial codominance; Lactic acid bacteria; Lactobacillus plantarum; Sourdough; Starter selection; TRITICUM-DURUM KERNELS; WHEAT BREAD; STRAINS; DIVERSITY; QUALITY; DIFFERENTIATION; MICROORGANISMS; IDENTIFICATION; PURIFICATION; BIODIVERSITY;
D O I
10.1016/j.fm.2015.04.011
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fifteen sourdoughs produced in western Sicily (southern Italy) were analysed by classical methods for their chemico-physical characteristics and the levels of lactic acid bacteria (LAB). pH and total titratable acidity (TTA) were mostly in the range commonly reported for similar products produced in Italy, but the fermentation quotient (FQ) of the majority of samples was above 4.0, due to the low concentration of acetic acid estimated by high performance liquid chromatography (HPLC). Specific counts of LAB showed levels higher than 108 CFU g(-1) for many samples. The colonies representing various morphologies were isolated and, after the differentiation based on phenotypic characteristics, divided into 10 groups. The most numerous group was composed of facultative heterofermentative isolates, indicating a relevance of this bacterial group during fermentation. The genetic analysis by randomly amplified polymorphic DNA (RAPD)-PCR, 16S rRNA gene sequencing and species-specific PCRs identified 33 strains as Lactobacillus plantarum, Lactobacillus curvatus and Lactobacillus graminis. Due to the consistent presence of L. plantarum, it was concluded that this species codominates with obligate heterofermentative LAB in sourdough production in this geographical area. In order to evaluate the performances at the basis of their fitness, the 29 L. plantarum strains were investigated for several technological traits. Twelve cultures showed good acidifying abilities in vitro and L. plantarum PON100148 produced the highest concentrations of organic acids. Eleven strains were positive for extracellular protease activity. Bacteriocin-like inhibitory substances (BLIS) production and antifungal activity was scored positive for several strains, included L. plantarum PON100148 which was selected as starter for experimental sourdough production. The characteristics of the sourdoughs and the resulting breads indicated that the best productions were obtained in presence of L. plantarum PON100148. (C) 2015 Elsevier Ltd. All rights reserved.
引用
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页码:57 / 68
页数:12
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