Regulated DNA rearrangement during sporulation in Bacillus weihenstephanensis KBAB4

被引:17
作者
Abe, Kimihiro [1 ]
Yoshinari, Akira [2 ]
Aoyagi, Takahiro [2 ]
Hirota, Yasunori [2 ]
Iwamoto, Keito [2 ]
Sato, Tsutomu [1 ,2 ]
机构
[1] Hosei Univ, Res Ctr Micronano Technol, Koganei, Tokyo 1840003, Japan
[2] Hosei Univ, Dept Frontier Biosci, Koganei, Tokyo 1848584, Japan
关键词
DEVELOPMENTAL TRANSCRIPTION FACTOR; SITE-SPECIFIC RECOMBINASE; DIPICOLINIC ACID; SUBTILIS SPORULATION; NUCLEOTIDE-SEQUENCE; GENE-EXPRESSION; RNA-POLYMERASE; CEREUS GROUP; SIGMA-K; SPORES;
D O I
10.1111/mmi.12375
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Temperate phages can integrate their genomes into a specific region of a host chromosome to produce lysogens (prophage). During genome insertion, prophages may interrupt the gene coding sequence. In Bacillus subtilis, the sigma factor gene sigK is interrupted by a 48kb prophage-like element. sigK is a composite coding sequence from two partial genes during sporulation. For over two decades, however, no further examples of DNA element-mediated gene reconstitution other than sigK have been identified in spore formers. Here we report that the gene for dipicolinic acid (DPA) synthetase subunit spoVFB in B.weihenstephanensisKBAB4 is interrupted by a prophage-like element named vfbin. DPA is synthesized in the mother cell and required for maintaining spore dormancy. We found that spoVFB was a composite coding sequence generated in the mother cell via chromosomal rearrangement that excised vfbin. Furthermore, vfbin caused excision after phage-inducer treatment, but vfbin appeared to be defective as a prophage. We also found various spore-forming bacteria in which sporulation-related genes were disrupted by prophage-like DNA elements. These results demonstrate the first example of a similar mechanism that affects a sporulation gene other than sigK and suggest that this prophage-mediated DNA rearrangement is a common phenomenon in spore-forming bacteria.
引用
收藏
页码:415 / 427
页数:13
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