Root Exudates from Grafted-Root Watermelon Showed a Certain Contribution in Inhibiting Fusarium oxysporum f. sp niveum

被引：88

作者：

Ling, Ning ^{[1
,2
]}

Zhang, Wenwen ^{[1
,2
]}

Wang, Dongsheng ^{[3
]}

Mao, Jiugeng ^{[3
]}

Huang, Qiwei ^{[1
,2
]}

Guo, Shiwei ^{[1
,2
]}

Shen, Qirong ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Jiangsu Key Lab, Minist Agr, Key Lab Plant Nutr & Fertilizat Low Middle Reache, Nanjing, Jiangsu, Peoples R China

[2] Nanjing Agr Univ, Engn Ctr Solid Organ Waste Utilizat, Nanjing, Jiangsu, Peoples R China

[3] Nanjing Inst Vegetable Sci, Nanjing, Jiangsu, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 06期

关键词：

BIOORGANIC FERTILIZER; VERTICILLIUM-DAHLIAE; PHENOLIC-ACIDS; CINNAMIC ACID; ROOTSTOCK; GROWTH; WILT; RESISTANCE; CUCUMBER; IDENTIFICATION;

D O I：

10.1371/journal.pone.0063383

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Grafting watermelon onto bottle gourd rootstock is commonly used method to generate resistance to Fusarium oxysporum f. sp. niveum (FON), but knowledge of the effect of the root exudates of grafted watermelon on this soil-borne pathogen in rhizosphere remains limited. To investigate the root exudate profiles of the own-root bottle gourd, grafted-root watermelon and own-root watermelon, recirculating hydroponic culture system was developed to continuously trap these root exudates. Both conidial germination and growth of FON were significantly decreased in the presence of root exudates from the grafted-root watermelon compared with the own-root watermelon. HPLC analysis revealed that the composition of the root exudates released by the grafted-root watermelon differed not only from the own-root watermelon but also from the bottle gourd rootstock plants. We identified salicylic acid in all 3 root exudates, chlorogenic acid and caffeic acid in root exudates from own-root bottle gourd and grafted-root watermelon but not own-root watermelon, and abundant cinnamic acid only in own-root watermelon root exudates. The chlorogenic and caffeic acid were candidates for potentiating the enhanced resistance of the grafted watermelon to FON, therefore we tested the effects of the two compounds on the conidial germination and growth of FON. Both phenolic acids inhibited FON conidial germination and growth in a dose-dependent manner, and FON was much more susceptible to chlorogenic acid than to caffeic acid. In conclusion, the key factor in attaining the resistance to Fusarium wilt is grafting on the non-host root stock, however, the root exudates profile also showed some contribution in inhibiting FON. These results will help to better clarify the disease resistance mechanisms of grafted-root watermelon based on plant-microbe communication and will guide the improvement of strategies against Fusarium-mediated wilt of watermelon plants.

引用

页数：8

共 40 条

[1]

Badri DV, 2009, PLANT CELL ENVIRON, V32, P666, DOI [10.1111/j.1365-3040.2008.01926.x, 10.1111/j.1365-3040.2009.01926.x]

[2] HPLC SEPARATION AND WAVELENGTH AREA RATIOS OF MORE THAN 50 PHENOLIC-ACIDS AND FLAVONOIDS [J].