Cryopreservation of Shoot Tips of Bacopa monnieri (L.) Wettst. by Vitrification Technique

被引：0
|
作者：
Sharma, N. [1 ]
Satsangi, R. [1 ]
Pandey, R. [1 ]
机构：
[1] Natl Bur Plant Genet Resources, Tissue Culture & Cryopreservat Unit, New Delhi 110012, India
来源：
INTERNATIONAL SYMPOSIUM ON CRYOPRESERVATION IN HORTICULTURAL SPECIES | 2011年 / 908卷
关键词：
Bacopa monnieri; Brahmi; cryopreservation; medicinal plant; memory vitalizer; vitrification; PVS2; ENDANGERED MEDICINAL-PLANT;
D O I：
暂无
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A protocol for cryopreservation of in vitro shoot tips of Bacopa monnieri (L.) Wettst., a medicinal plant of high commercial value was developed. Shoot tips (about 1 mm in length) excised from 4- to 24-week-old proliferating shoot cultures were precultured on MS medium supplemented with various cryoprotectants before dehydration with PVS2 at 0 degrees C. The dehydrated shoot tips were directly immersed in liquid N-2. Following cryopreservation, and after rapid rewarming at 45 degrees C, shoot tips were quickly washed with 1.2 M sucrose solution and then plated on solidified shoot culture medium. Shoot tips were successfully cryopreserved by vitrification, when they were precultured on medium supplemented with 5% DMSO for two days before dehydration. Preculture of shoot tips with sucrose at 25 degrees C significantly improved the survival and shoot regeneration rate of cryopreserved shoot tips. Regenerated plantlets were successfully transferred to pots.
引用
收藏
页码：283 / 288
页数：6
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