N-(2-ferrocene-ethyl)maleimide: A new electroactive sulphydryl-specific reagent for cysteine-containing peptides and proteins

被引：42

作者：

DiGleria, K ^{[1
]}

Hill, HAO ^{[1
]}

Wong, LL ^{[1
]}

机构：

[1] UNIV OXFORD, NEW CHEM LAB, OXFORD OX1 3QT, ENGLAND

来源：

FEBS LETTERS | 1996年 / 390卷 / 02期

关键词：

electroactive label; sulfhydryl-specific reagent; cytochrome P-450; glutathione; electrochemistry;

D O I：

10.1016/0014-5793(96)00648-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the synthesis and application of a specific electroactive label, N-(2-ferrocene-ethyl)maleimide, which provides nem redox properties to organic compounds and proteins possessing sulphydryl groups, Its reaction conditions with the cysteine-containing peptide, glutathione, and a terminal monooxygenase enzyme, cytochrome P450(cam) are presented. The labelled peptide and enzyme acquired reversible electrochemical properties due to the attached ferrocene moiety.

引用

页码：142 / 144

页数：3

共 16 条

[1]

ATKINS WA, 1971, ARCH BIOCHEM BIOPHYS, V144, P678

[2]

ATKINS WM, 1988, J BIOL CHEM, V263, P18842

[3] DISTANCE DEPENDENCE OF PHOTOINDUCED LONG-RANGE ELECTRON-TRANSFER IN ZINC RUTHENIUM-MODIFIED MYOGLOBINS [J].