Visible-Light-Mediated Thiol-Ene Hydrogelation Using Eosin-Y as the Only Photoinitiator

被引：172

作者：

Shih, Han ^{[1
]}

Lin, Chien-Chi ^{[1
]}

机构：

[1] Indiana Univ Purdue Univ, Dept Biomed Engn, Indianapolis, IN 46202 USA

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2013年 / 34卷 / 03期

关键词：

cell encapsulation; eosin-Y; hydrogels; photopolymerization; thiol-ene chemistry; POLY(ETHYLENE GLYCOL) DIACRYLATE; CLICK-BASED HYDROGELS; INTERFACIAL PHOTOPOLYMERIZATION; CELL ENCAPSULATION; CROSS-LINKING; CHEMISTRY; CYTOCOMPATIBILITY; PROTEIN; UV;

D O I：

10.1002/marc.201200605

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The utility of visible-light-mediated polymerization in tissue engineering has been limited due to the necessary use of potentially cytotoxic coinitiator and comonomer. Here, we report a visible-light-mediated thiol-ene hydrogelation scheme using eosin-Y as the only photoinitiator. Under visible light exposure, rapid and highly tunable step-growth gelation is achieved using PEG-norbornene and a model cross-linker dithiothreitol. In addition to investigating the gelation kinetics and properties of thiol-ene hydrogels formed by this new gelation scheme, we also report high cytocompatibility of these hydrogels using human mesenchymal stem cells (hMSCs) and pancreatic MIN6 beta-cells.

引用

页码：269 / 273

页数：5

共 29 条

[1] The performance of human mesenchymal stem cells encapsulated in cell-degradable polymer-peptide hydrogels [J].

Anderson, Sarah B. ;

Lin, Chien-Chi ;

Kuntzler, Donna V. ;

Anseth, Kristi S. .

BIOMATERIALS, 2011, 32 (14) :3564-3574

[2] VISIBLE LIGHT PHOTOINITIATION OF MESENCHYMAL STEM CELL-LADEN BIORESPONSIVE HYDROGELS [J].

Bahney, C. S. ;

Lujan, T. J. ;

Hsu, C. W. ;

Bottlang, M. ;

West, J. L. ;

Johnstone, B. .

EUROPEAN CELLS & MATERIALS, 2011, 22 :43-55

[3] Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels [J].

Benton, Julie A. ;

Fairbanks, Benjamin D. ;

Anseth, Kristi S. .

BIOMATERIALS, 2009, 30 (34) :6593-6603

[4] Cytocompatibility of UV and visible light photoinitiating systems on cultured NIH/3T3 fibroblasts in vitro [J].

Bryant, SJ ;

Nuttelman, CR ;

Anseth, KS .

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2000, 11 (05) :439-457

[5]

Bryant SJ., 2005, Scaffolding in Tissue Engineering, chapter Photopolymerization of Hydrogel Scaffolds

[6]

Cruise GM, 1998, BIOTECHNOL BIOENG, V57, P655, DOI 10.1002/(SICI)1097-0290(19980320)57:6<655::AID-BIT3>3.0.CO

[7]

2-K

[8] Photoreversible Patterning of Biomolecules within Click-Based Hydrogels [J].

DeForest, Cole A. ;

Anseth, Kristi S. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (08) :1816-1819

[9]

DeForest CA, 2011, NAT CHEM, V3, P925, DOI [10.1038/NCHEM.1174, 10.1038/nchem.1174]

[10] Conjugate addition reactions combined with free-radical cross-linking for the design of materials for tissue engineering [J].

Elbert, DL ;

Hubbell, JA .

BIOMACROMOLECULES, 2001, 2 (02) :430-441

← 1 2 3 →