Coding sequence of a hyaluronan synthase homologue expressed during expansion of the mouse cumulus-oocyte complex

被引:128
作者
Fulop, C [1 ]
Salustri, A [1 ]
Hascall, VC [1 ]
机构
[1] UNIV ROMA TOR VERGATA,DIPARTIMENTO SANITA PUBBL & BIOL CELLULARE,I-00173 ROME,ITALY
关键词
hyaluronan synthase; cumulus cell-oocyte complex;
D O I
10.1006/abbi.1996.9793
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Maturation of mammalian cumulus cell-oocyte complex in the preovulatory follicle involves the deposition of a hyaluronan-rich extracellular matrix by the cumulus cells. in this study, we report the complete coding sequence of a novel mouse hyaluronan synthase homologue expressed in cumulus cell-oocyte complexes induced to expand in vivo. The time frame of mRNA expression of this molecule correlates well with previous biochemical and immunohistochemical findings on the initiation of hyaluronan synthesis in maturing preovulatory follicles. Evolutionary comparison of the vertebrate hyaluronan synthase homologues indicates that there are at least two genes that code for these proteins. (C) 1997 Academic Press
引用
收藏
页码:261 / 266
页数:6
相关论文
共 29 条
[1]  
[Anonymous], 1978, ALTAS PROTEIN SEQUEN
[2]  
DEANGELIS PL, 1993, J BIOL CHEM, V268, P19181
[3]   MATURATION OF THE RAT CUMULUS OOPHORUS - SCANNING ELECTRON-MICROSCOPIC STUDY [J].
DEKEL, N ;
PHILLIPS, DM .
BIOLOGY OF REPRODUCTION, 1979, 21 (01) :9-18
[4]   ANALYSIS OF MEMBRANE AND SURFACE PROTEIN SEQUENCES WITH THE HYDROPHOBIC MOMENT PLOT [J].
EISENBERG, D ;
SCHWARZ, E ;
KOMAROMY, M ;
WALL, R .
JOURNAL OF MOLECULAR BIOLOGY, 1984, 179 (01) :125-142
[6]  
FERAMISCO JR, 1980, J BIOL CHEM, V255, P4240
[7]   CONSTRUCTION OF PHYLOGENETIC TREES [J].
FITCH, WM ;
MARGOLIASH, E .
SCIENCE, 1967, 155 (3760) :279-+
[8]   PREDICTING THE ORIENTATION OF EUKARYOTIC MEMBRANE-SPANNING PROTEINS [J].
HARTMANN, E ;
RAPOPORT, TA ;
LODISH, HF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (15) :5786-5790
[9]   Expression cloning and molecular characterization of HAS protein, a eukaryotic hyaluronan synthase [J].
Itano, N ;
Kimata, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (17) :9875-9878
[10]   Molecular cloning of human hyaluronan synthase [J].
Itano, N ;
Kimata, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 222 (03) :816-820