Matrine- and oxymatrine-imprinted monodisperse polymers prepared by precipitation polymerization and their applications for the selective extraction of matrine-type alkaloids from Sophora flavescens Aiton

被引:44
作者
Funaya, Noriko [1 ]
Haginaka, Jun [1 ]
机构
[1] Mukogawa Womens Univ, Sch Pharm & Pharmaceut Sci, Nishinomiya, Hyogo 6638179, Japan
关键词
Molecularly imprinted polymer; Precipitation polymerization; Matrine; Oxymatrine; Sophocarpine; Oxysophocarpine; QUINOLIZIDINE ALKALOIDS; LIQUID-CHROMATOGRAPHY; SEPARATION; ACIDS;
D O I
10.1016/j.chroma.2012.05.081
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Matrine (MT)- and oxymatrine (OMT)-imprinted monodisperse polymers have been prepared by precipitation polymerization. The prepared molecularly imprinted polymers (MIPs) for MT and OMT, MIPMT and MIPOMT, were monodispersed microspheres of 3.3 and 3.9 mu m in diameter, respectively. Binding experiments and Scatchard analyses revealed that two classes of binding sites were formed on MIPMT and MIPOMT. In addition to shape recognition, ionic and hydrophobic interactions seemed to affect the retention and recognition of MT and OMT on MIPMT and MIPOMT, respectively, in low acetonitrile content, and ionic and hydrophilic interactions affected these properties in high acetonitrile content. MIPMT was used to selectively extract MT and sophocarpine (13,14-dehydromatrine) from Sophora flavescens root, while MIPOMT was used to extract OMT and oxysophocarpine (13,14-dehydrooxymatrine). (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:18 / 23
页数:6
相关论文
共 23 条
[1]   HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].
ALPERT, AJ .
JOURNAL OF CHROMATOGRAPHY, 1990, 499 :177-196
[2]   Solute attributes and molecular interactions contributing to "U-shape" retention on a fluorinated high-performance liquid chromatography stationary phase [J].
Bell, DS ;
Jones, AD .
JOURNAL OF CHROMATOGRAPHY A, 2005, 1073 (1-2) :99-109
[3]   SEPARATION OF ALKALOIDS FROM DATURA-METE L AND SOPHORA-FLAVESCENS AIT BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY [J].
CAI, DG ;
GU, MJ ;
ZHANG, JD ;
ZHU, GP ;
ZHANG, TY ;
LI, N ;
ITO, Y .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1990, 13 (12) :2399-2408
[4]   Recent advances in molecular imprinting technology: current status, challenges and highlighted applications [J].
Chen, Lingxin ;
Xu, Shoufang ;
Li, Jinhua .
CHEMICAL SOCIETY REVIEWS, 2011, 40 (05) :2922-2942
[5]   Liquid chromatography of active principles in Sophora flavescens root [J].
Chen, X ;
Yi, CQ ;
Yang, XQ ;
Wang, XR .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 812 (1-2) :149-163
[6]   A novel route to new carbonyl derivatives of cinchonine and cinchonidine [J].
Dodin, G ;
Cordier, C ;
Menager, L ;
Bourzegue, A ;
Blais, JC .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1998, (21) :3619-3621
[7]   Matrine-imprinted monolithic stationary phase for extraction and purification of matrine from Sophorae flavescentis Ait [J].
Fu, Qiang ;
Fang, Qi ;
Feng, Bianling ;
Sun, Sijuan ;
Du, Wei ;
Amut, Enijian ;
Xiao, Aiping ;
Chang, Chun .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (13-14) :894-900
[8]   Molecularly imprinted solid-phase extraction of matrine from radix Sophorae tonkinensis [J].
Guo, Zhifeng ;
Zhang, Lixia ;
Song, Caiyu ;
Zhang, Xiaoxuan .
ANALYST, 2011, 136 (14) :3016-3022
[9]   Retentivity and enantioselectivity of uniformly sized molecularly imprinted polymers for d-chlorpheniramine and -brompheniramine in hydro-organic mobile phases [J].
Haginaka, J ;
Kagawa, C .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 804 (01) :19-24
[10]   Chiral resolution of derivatized amino acids using uniformly sized molecularly imprinted polymers in hydro-organic mobile phases [J].
Haginaka, J ;
Kagawa, C .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2004, 378 (08) :1907-1912