Droplet-based bioprinting enables the fabrication of cell-hydrogel-microfibre composite tissue precursors

被引:26
作者
Kotlarz, Marcin [1 ]
Ferreira, Ana M. [1 ]
Gentile, Piergiorgio [1 ]
Russell, Stephen J. [2 ]
Dalgarno, Kenneth [1 ]
机构
[1] Newcastle Univ, Sch Engn, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[2] Univ Leeds, Sch Design, Leeds LS2 9JT, W Yorkshire, England
基金
英国工程与自然科学研究理事会;
关键词
Bioprinting; Hydrogel-fibre composites; High-cell-density hydrogels; Composite manufacturing; ELECTROSPUN-FIBERS; ALGINATE HYDROGELS; NONWOVEN SCAFFOLDS; WOUND DRESSINGS; SKIN; KERATINOCYTES; BIOMATERIALS; STRATEGIES; CALCIUM; DESIGN;
D O I
10.1007/s42242-022-00192-5
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Composites offer the option of coupling the individual benefits of their constituents to achieve unique material properties, which can be of extra value in many tissue engineering applications. Strategies combining hydrogels with fibre-based scaffolds can create tissue constructs with enhanced biological and structural functionality. However, developing efficient and scalable approaches to manufacture such composites is challenging. Here, we use a droplet-based bioprinting system called reactive jet impingement (ReJI) to integrate a cell-laden hydrogel with a microfibrous mesh. This system uses microvalves connected to different bioink reservoirs and directed to continuously jet bioink droplets at one another in mid-air, where the droplets react and form a hydrogel that lands on a microfibrous mesh. Cell-hydrogel-fibre composites are produced by embedding human dermal fibroblasts at two different concentrations (5 x 10(6) and 30 x 10(6) cells/mL) in a collagen-alginate-fibrin hydrogel matrix and bioprinted onto a fibre-based substrate. Our results show that both types of cell-hydrogel-microfibre composite maintain high cell viability and promote cell-cell and cell-biomaterial interactions. The lower fibroblast density triggers cell proliferation, whereas the higher fibroblast density facilitates faster cellular organisation and infiltration into the microfibres. Additionally, the fibrous component of the composite is characterised by high swelling properties and the quick release of calcium ions. The data indicate that the created composite constructs offer an efficient way to create highly functional tissue precursors for laminar tissue engineering, particularly for wound healing and skin tissue engineering applications. [GRAPHICS] .
引用
收藏
页码:512 / 528
页数:17
相关论文
共 65 条
[1]   Alginate in Wound Dressings [J].
Aderibigbe, Blessing Atim ;
Buyana, Buhle .
PHARMACEUTICS, 2018, 10 (02)
[2]   Hydrogel: Preparation, characterization, and applications: A review [J].
Ahmed, Enas M. .
JOURNAL OF ADVANCED RESEARCH, 2015, 6 (02) :105-121
[3]   In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds [J].
Albanna, Mohammed ;
Binder, Kyle W. ;
Murphy, Sean V. ;
Kim, Jaehyun ;
Qasem, Shadi A. ;
Zhao, Weixin ;
Tan, Josh ;
El-Amin, Idris B. ;
Dice, Dennis D. ;
Marco, Julie ;
Green, Jason ;
Xu, Tao ;
Skardal, Aleksander ;
Holmes, James H. ;
Jackson, John D. ;
Atala, Anthony ;
Yoo, James J. .
SCIENTIFIC REPORTS, 2019, 9 (1)
[4]   Alginate hydrogels as biomaterials [J].
Augst, Alexander D. ;
Kong, Hyun Joon ;
Mooney, David J. .
MACROMOLECULAR BIOSCIENCE, 2006, 6 (08) :623-633
[5]  
Avila Hector Martinez, 2016, Bioprinting, V1-2, P22, DOI 10.1016/j.bprint.2016.08.003
[6]   A two-layer skin construct consisting of a collagen hydrogel reinforced by a fibrin-coated polylactide nanofibrous membrane [J].
Bacakova, Marketa ;
Pajorova, Julia ;
Broz, Antonin ;
Hadraba, Daniel ;
Lopot, Frantisek ;
Zavadakova, Anna ;
Vistejnova, Lucie ;
Beno, Milan ;
Kostic, Ivan ;
Jencova, Vera ;
Bacakova, Lucie .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2019, 14 :5033-5050
[7]  
Benning MJ, 2019, Printing Apparatus and Method, Patent No. [WO/2019/008373, 2019008373]
[8]   State of the art composites comprising electrospun fibres coupled with hydrogels: a review [J].
Bosworth, Lucy A. ;
Turner, Lesley-Anne ;
Cartmell, Sarah H. .
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2013, 9 (03) :322-335
[9]   Three-dimensional printed electrospun fiber-based scaffold for cartilage regeneration [J].
Chen, Weiming ;
Xu, Yong ;
Liu, Yanqun ;
Wang, Zongxin ;
Li, Yaqiang ;
Jiang, Gening ;
Mo, Xiumei ;
Zhou, Guangdong .
MATERIALS & DESIGN, 2019, 179
[10]   Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution [J].
Chong, E. J. ;
Phan, T. T. ;
Lim, I. J. ;
Zhang, Y. Z. ;
Bay, B. H. ;
Ramakrishna, S. ;
Lim, C. T. .
ACTA BIOMATERIALIA, 2007, 3 (03) :321-330