Toehold-initiated Rolling Circle Amplification for Visualizing Individual MicroRNAs In Situ in Single Cells

被引：407

作者：

Deng, Ruijie ^{[1
]}

Tang, Longhua ^{[1
]}

Tian, Qianqian ^{[1
]}

Wang, Ying ^{[1
]}

Lin, Lei ^{[1
]}

Li, Jinghong ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem, Beijing Key Lab Microanalyt Methods & Instrumenta, Beijing 100084, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 09期

基金：

中国国家自然科学基金;

关键词：

in situ imaging; isothermal amplification; microRNAs; RNA structures; strand displacement; NUCLEIC-ACID PROBES; NUCLEOTIDE POLYMORPHISMS; DNA LIGATION; RNA; HYBRIDIZATION; EXPRESSION;

D O I：

10.1002/anie.201309388

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The ability to quantitate and visualize microRNAs (miRNAs) in situ in single cells would greatly facilitate the elucidation of miRNA-mediated regulatory circuits and their disease associations. A toehold-initiated strand-displacement process was used to initiate rolling circle amplification of specific miRNAs, an approach that achieves both stringent recognition and in situ amplification of the target miRNA. This assay, termed toehold-initiated rolling circle amplification (TIRCA), can be utilized to identify miRNAs at physiological temperature with high specificity and to visualize individual miRNAs in situ in single cells within 3 h. TIRCA is a competitive candidate technique for in situ miRNA imaging and may help us to understand the role of miRNAs in cellular processes and human diseases in more detail.

引用

页码：2389 / 2393

页数：5

共 52 条

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