Changes in S1P1 and SIP2 expression during embryonal development and primitive endoderm differentiation of F9 cells

被引：6

作者：

Hiraga, Yuki ^{[1
]}

Kibara, Akio ^{[1
]}

Sano, Takamitsu ^{[1
]}

Igarashi, Yasuyuki ^{[1
]}

机构：

[1] Hokkaido Univ, Dept Biomembrane & Biofunct Chem, Grad Sch Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 03期

关键词：

sphingolipid; sphingosine; 1-phosphate; SIP receptor; SIP1; embryonal development; differentiation; endoderm; F9; cells;

D O I：

10.1016/j.bbrc.2006.04.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sphingosine 1-phosphate (SIP) is a ligand for SIP family receptors (S1P(1)-S1P(5)). Of these receptors, S1P(1), S1P2, and S1P(3) are ubiquitously expressed in adult mice, while S1P(4) and S1P(5) are tissue specific. However, little is known of their expression during embryonal development. We performed Northern blot analyses in mouse embryonal tissue and found that such expression is developmentally regulated. We also examined the expression of these receptors during primitive endoderm (PrE) differentiation of mouse F9 embryonal carcinoma (EC) cells, a well-known in vitro endoderm differentiation system. S1P(2) mRNA was abundantly expressed in F9 EC cells, but little S1P(1) and no S1P(3), S1P(4), or S1P(5) mRNA was detectable. However, S1P(1) mRNA expression was induced during EC-to-PrE differentiation. Studies using small interference RNA of S1P(1) indicated that increased SIP, expression is required for PrE differentiation. Thus, SIP, may play an important function in PrE differentiation that is not substituted for by SIP,. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：852 / 858

页数：7

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