Arsenic trioxide reduces the invasive and metastatic properties of nasopharyngeal carcinoma cells in vitro

被引:13
作者
Du, C. W.
Wen, B. G.
Li, D. R.
Peng, X.
Hong, C. Q.
Chen, J. Y.
Lin, Z. Z.
Hong, X.
Lin, Y. C.
Xie, L. X.
Wu, M. Y.
Zhang, H.
机构
[1] Shantou Univ, Coll Med, Canc Hosp, Canc Res Lab, Guangdong 515031, Peoples R China
[2] Shantou Univ, Coll Med, Canc Hosp, Dept Radiotherapy, Guangdong 515031, Peoples R China
[3] Shantou Univ, Coll Med, Dept Canc Mol Biol, Guangdong 515031, Peoples R China
[4] Shantou Univ, Coll Med, Dept Pathol, Guangdong 515031, Peoples R China
[5] Linkoping Univ, Clin Res Ctr, Div Dermatol, Dept Biomed & Surg, Linkoping, Sweden
关键词
arsenic trioxide (AS(2)O(3)); nasopharyngeal carcinoma; metastases; latent membrane protein 1; metalloproteinase; 9;
D O I
10.1590/S0100-879X2006000500015
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nasopharyngeal carcinoma (NPC) is notorious for the metastases, which are in close association with Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). Arsenic trioxide (AS(2)O(3)) has been shown to induce apoptosis and differentiation in NPC xenografts. Then, can it repress the cancer cells' metastasis potential? To elucidate this issue, the present study was performed. LMP1-negative cell line HNE1 and LMP1-positive cell line HNE1-LMP1 were used as in vitro model. Cells (1 x 10(5)/mL) were cultured with or without 3 mu M AS(2)O(3) for 48 h. Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. Both confocal immunofluorescence staining and Western blot were used to detect the changes of LMP1 expression. The changes of MMP-9 were examined by RT-PCR assay and Western blot. The results were as follow: i) the colony formation inhibition rate (75.41 +/- 3.9% in HNE1-LMP1 cells vs 37.89 +/- 4.9% in HNE1 cells), the rate of attachment (HNE1-LMT1 vs HNE1: 56.40 +/- 3.5 vs 65.87 +/- 5.9%), the invasion inhibitory rate (HNE1-LW1 vs HNE1: 56.50 +/- 3.7 and 27.91 +/- 2.1%), and the migration inhibitory rate (HNE1-LMP1 vs HNE1: 48.70 +/- 3.9 vs 29.19 +/- 6.27%) were all significantly different between the two cell lines (P < 0.01). ii) LMP1 was down-regulated in AS(2)O(3)-treated HNE1-LMP1 cells. iii) The reduction of MMP-9 was found in AS(2)O(3)-treated groups, more evident in HNE1-LMP1 cells. Thus, we conclude that AS(2)O(3) can reduce metastasis potential of NPC cells, involving inhibition of MW-9 expression. LMP1 were also reduced in this process and seemed to enhance anti-metastasis activity of AS(2)O(3).
引用
收藏
页码:677 / 685
页数:9
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