Immune Response of Gnotobiotic Piglets against Mycoplasma hyopneumoniae

被引：28

作者：

Muneta, Yoshihiro ^{[1
]}

Minagawa, Yu ^{[1
]}

Shimoji, Yoshihiro ^{[1
]}

Ogawa, Yohsuke ^{[1
]}

Hikono, Hirokazu ^{[1
]}

Mori, Yasuyuki ^{[2
]}

机构：

[1] Natl Inst Anim Hlth, Res Team Adv Biol, Tsukuba, Ibaraki 3050856, Japan

[2] Natl Inst Anim Hlth, Res Team Paratb, Tsukuba, Ibaraki 3050856, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2008年 / 70卷 / 10期

关键词：

cytokine; gnotobiotic; immunomodulation; Mycoplasma hyopneumoniae; swine;

D O I：

10.1292/jvms.70.1065

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In this study. several cytokine responses were investigated during Mycoplasma hyopneumoniae (Mhp) infection using a gnobiotic infection model. We found that several inflammatory cytokines (IL-1 beta, IL-8, IL-18, and TNF-alpha) and an anti-inflammatory cytokine IL-10 were induced from peripheral blood mononuclear cells (PBMC) of germ-free (GF) piglets Stimulated with heat killed Mhp Whole antigens, but no IFN-gamma and IL-4 were induced by Mhp. After the intranasal infection of Mhp, IL-1 beta, IL-8. IL-18, and IFN-gamma were also detected in the broncho-alveolar lavage fluids (BALF). The antigen-specific IFN-gamma and IL-10 responses after infection of Mhp were gradually suppressed during Mhp infection as well as non-specific immune response to concanavalin A (ConA) and lipopolysacchalide (LPS) at eariv stage of infection. These results suggested that Mhp infection modulates the immune response of pigs by inducing several cytokines, and causes immuno-suppresion of pigs in a gnotobiotic condition.

引用

页码：1065 / 1070

页数：6

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