Polylactosaminoglycan Glycomics: Enhancing the Detection of High-molecular-weight N-glycans in Matrix-assisted Laser Desorption Ionization Time-of-flight Profiles by Matched Filtering

被引:15
作者
Bern, Marshall [1 ]
Brito, Alejandro E. [1 ]
Pang, Poh-Choo [2 ]
Rekhi, Angad [2 ]
Dell, Anne [2 ]
Haslam, Stuart M. [2 ]
机构
[1] Palo Alto Res Ctr, Palo Alto, CA 94304 USA
[2] Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, London SW7 2AZ, England
基金
英国生物技术与生命科学研究理事会;
关键词
MASS-SPECTROMETRIC ANALYSIS; CARBOHYDRATE MOIETY; LACTOSAMINOGLYCAN; GLYCOSYLATION; IDENTIFICATION; GLYCOPROTEINS; EXPRESSION;
D O I
10.1074/mcp.O112.026377
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
For over 30 years, protocols based on the mass spectrometry (MS) of permethylated derivatives, complemented by enzymatic degradations, have underpinned glycomic experiments aimed at defining the structures of individual glycans present in the complex mixtures that are characteristic of biological samples. Both MS instrumentation and sample handling have improved markedly in recent years, enabling greater sensitivity and better signal-to-noise ratios, thereby facilitating the detection of glycans at much higher masses than could be achieved in the past. The latter is especially important for the characterization of the biologically important class of N-glycans that carry polylactosaminoglycan chains. Such advances in data acquisition heighten the need for informatics tools to assist in glycan structure assignment. Here, utilizing mouse lung tissue as a model system, we present evidence of polylactosaminoglycan-containing N-glycans with permethylated molecular weights exceeding 13 kDa. We show that antennae branching patterns and lengths can be successfully determined at these high masses via MS/MS experiments, even when MS ion counts are very low. We also describe the development and application of a matched filtering algorithm for assisting high-molecular-weight glycan detection and structure assignment. Molecular & Cellular Proteomics 12: 10.1074/mcp.O112.026377, 996-1004, 2013.
引用
收藏
页码:996 / 1004
页数:9
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