Batch and continuous hydrolysis of lactose using β-galactosidase immobilized on gelatin-CMC

被引：9

作者：

Sungur, S ^{[1
]}

Yildirim, Ö

机构：

[1] Ankara Univ, Fac Sci, Dept Chem, TR-06100 Ankara, Turkey

[2] Ankara Univ, Fac Sci, Dept Biol, TR-06100 Ankara, Turkey

来源：

POLYMER-PLASTICS TECHNOLOGY AND ENGINEERING | 1999年 / 38卷 / 04期

关键词：

D O I：

10.1080/03602559909351616

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

beta-Galactosidase enzyme (E,C. 3.2.1.23) was immobilized onto a gelatin-carboxymethylcellulose carrier system by using cross-linker chromium acetate, A relative activity increase of 33% was achieved by adding carboxymethylcellulose to gelatin, The effect of operation temperature and pH on relative activity and reusability of the immobilized enzyme was investigated, A fluidized-bed reactor with a downflow mechanism was used for continuous process hydrolysis, and a constant glucose production rate was obtained.

引用

页码：821 / 829

页数：9

共 50 条

[31] Repeated-Batch Operation of Immobilized β-Galactosidase Inclusion Bodies-Containing Escherichia coli Cell Reactor for Lactose Hydrolysis
Yeon, Ji-Hyeon
Jung, Kyung-Hwan
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 21 (09) : 972 - 978
[32] High stability of immobilized β-D-galactosidase for lactose hydrolysis and galactooligosaccharides synthesis
Klein, Manuela P.
Fallavena, Lucas P.
Schoeffer, Jessie da N.
Ayub, Marco A. Z.
Rodrigues, Rafael C.
Ninow, Jorge L.
Hertz, Plinho F.
CARBOHYDRATE POLYMERS, 2013, 95 (01) : 465 - 470
[33] HYDROLYSIS OF LACTOSE BY A THERMOSTABLE BETA-GALACTOSIDASE IMMOBILIZED ON DEAE-CELLULOSE
GRIFFITHS, MW
MUIR, DD
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1980, 31 (04) : 397 - 404
[34] A method for evaluating lactose hydrolysis in a fixed bed reactor with β-galactosidase immobilized on chitosan
Inst. Desarrollo Tecn. Indust. Q., Consejo Natl. Invest. Cie. T., Universidad Nacional del Litoral, Güemes 3450, 3000 Santa Fe, Argentina
Chem. Eng. J., 2 (93-98):
[35] Lactose hydrolysis using β-galactosidase from Kluyveromyces lactis immobilized with sodium alginate for potential industrial applications
de Carvalho, Catherine Teixeira
de Brito Lima, Wildson Bernardino
Macedo de Medeiros, Fabio Goncalves
de Medeiros Dantas, Julia Maria
de Araujo Padilha, Carlos Eduardo
dos Santos, Everaldo Silvino
de Macedo, Gorete Ribeiro
de Sousa Junior, Francisco Caninde
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2021, 51 (07) : 714 - 722
[36] Optimization and modeling of lactose hydrolysis in a packed bed system using immobilized β-galactosidase from Aspergillus oryzae
Fischer, Janaina
Guidini, Carla Zanella
Soares Santana, Larissa N.
de Resende, Miriam Maria
Cardoso, Vicelma Luiz
Ribeiro, Eloizio Julio
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2013, 85-86 : 178 - 186
[37] Studies on modelling and simulation of lactose hydrolysis by free and immobilized β-galactosidase from aspergillus niger
Papayannakos, N., 1600, (52):
[38] HYDROLYSIS OF LACTOSE IN SKIM MILK BY IMMOBILIZED BETA-GALACTOSIDASE (BACILLUS-CIRCULANS)
BAKKEN, AP
HILL, CG
AMUNDSON, CH
BIOTECHNOLOGY AND BIOENGINEERING, 1992, 39 (04) : 408 - 417
[39] EFFECTS OF TEMPERATURE ON THE HYDROLYSIS OF LACTOSE BY IMMOBILIZED BETA-GALACTOSIDASE IN A CAPILLARY BED REACTOR
PETERSON, RS
HILL, CG
AMUNDSON, CH
BIOTECHNOLOGY AND BIOENGINEERING, 1989, 34 (04) : 429 - 437
[40] HYDROLYSIS OF LACTOSE IN SKIM MILK BY IMMOBILIZED BETA-GALACTOSIDASE IN A SPIRAL FLOW REACTOR
BAKKEN, AP
HILL, CG
AMUNDSON, CH
BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (10) : 1249 - 1257

← 1 2 3 4 5 →