Batch and continuous hydrolysis of lactose using β-galactosidase immobilized on gelatin-CMC

beta-Galactosidase enzyme (E,C. 3.2.1.23) was immobilized onto a gelatin-carboxymethylcellulose carrier system by using cross-linker chromium acetate, A relative activity increase of 33% was achieved by adding carboxymethylcellulose to gelatin, The effect of operation temperature and pH on relative activity and reusability of the immobilized enzyme was investigated, A fluidized-bed reactor with a downflow mechanism was used for continuous process hydrolysis, and a constant glucose production rate was obtained.