Generation of multicellular tumor spheroids of breast cancer cells: How to go three-dimensional

被引：51

作者：

Nagelkerke, Anika ^{[1
,2
]}

Bussink, Johan ^{[1
]}

Sweep, Fred C. G. J. ^{[2
]}

Span, Paul N. ^{[1
,2
]}

机构：

[1] Radboud Univ Nijmegen, Dept Radiat Oncol, Med Ctr, NL-6500 HB Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Dept Lab Med, Med Ctr, NL-6500 HB Nijmegen, Netherlands

来源：

ANALYTICAL BIOCHEMISTRY | 2013年 / 437卷 / 01期

关键词：

Breast cancer cells; Spheroid culture; Hanging drop; Liquid overlay; Matrigel; MODEL; CULTURES; 3-D;

D O I：

10.1016/j.ab.2013.02.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The multicellular tumor spheroid model is a widely used culture system to mimic the three-dimensionality of tumors. Several methods and an even larger number of protocols are available to prepare spheroids from regular monolayers. This paper describes the assessment of several techniques frequently used to culture spheroids of breast cancer cells. We found that some methods produced suboptimal results. Ultimately, an adapted liquid overlay technique generated tight, compact and robust spheroids of the breast cancer cells tested. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：17 / 19

页数：3

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