Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)

被引:9
作者
Zhao, Da Qiu [1 ]
Han, Chen Xia [1 ]
Ge, Jin Tao [1 ]
Tao, Jun [1 ]
机构
[1] Yangzhou Univ, Coll Hort & Plant Protect, Jiangsu Key Lab Crop Genet & Physiol, Yangzhou, Peoples R China
来源
ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2012年 / 15卷 / 06期
关键词
anthocyanin; expression; glucosyltransferase; herbaceous peony; 3-O-GLUCOSYLTRANSFERASE UFGT GENE; MOLECULAR CHARACTERIZATION; FLOWERS; CLONING; GLUCOSYLTRANSFERASES; ENZYME;
D O I
10.2225/vol15-issue6-fulltext-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PIPSY, PICHS, PICHI, PIF3H, PIF3'H, PIDFR, PIANS, PIUF3GT and PIUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PIDFR and PIUF3GT, which could be the candidates to regulate P. lactiflora flower colour by means of genetic engineering.
引用
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页数:9
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