CLEIA CA125 evidences: good analytical performance avoiding "Hook effect"

被引:7
作者
Falzarano, R. [1 ]
Viggiani, V. [1 ]
Michienzi, S. [1 ]
Colaprisca, B. [1 ]
Longo, F. [1 ]
Frati, L. [1 ]
Anastasi, E. [1 ]
机构
[1] Univ Roma La Sapienza, Dept Mol Med, I-00161 Rome, Italy
关键词
CA125; biomarker; Radioimmunoassay; Chemiluminescence system; EPITHELIAL OVARIAN-CANCER; TUMOR-MARKERS; CA-125; ANTIGEN; GUIDELINES; ANTIBODY; MUCIN; ASSAY; MUC16; RISK;
D O I
10.1007/s13277-012-0561-6
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Cancer antigen 125 (CA125) is a coelomic epithelium-related antigen carried by a high molecular weight glycoprotein complex. It is commonly used as a tumor marker for ovarian cancer to monitor disease progression and response to therapy and as an early detection for recurrence after treatment. The aim of this study was to test the reliability of two different assay methods, a radioimmunometric assay (RIA) and an automated chemiluminescent enzyme immunoassay (CLEIA) system, by measuring CA125 serum levels using both methods in 357 patients and comparing the results. Patients were recruited from Oncologic Unit A, Policlinico Umberto I, Roma. Eighty-six were healthy donors, while 271 were oncologic patients representing a variety of diagnoses. Within this group, 76 patients were diagnosed with an ovarian related pathology (28 cancerous and 48 benign). The evaluation of CA125 marker blood levels showed a high agreement in healthy donors group (R (2) = 0.9003). Interesting results emerged when sera collected from oncologic patients were assessed: significant differences between the two assays were found in nine samples. When assayed again with RIA after a dilution, new values agreed with undiluted CLEIA values (R (2) = 0.9847). Our data suggest an overall good comparison between the two methods. However, some artifacts were obtained with RIA and indicate an underlying presence of "hook effect". CLEIA automated assay showed a good reliability and should be preferred to one-step radioimmunoassays in order to minimize errors.
引用
收藏
页码:387 / 393
页数:7
相关论文
共 26 条
  • [1] HE4 in the Differential Diagnosis of a Pelvic Mass: A Case Report
    Anastasi, Emanuela
    Granato, Teresa
    Coppa, Anna
    Manganaro, Lucia
    Giannini, Giuseppe
    Comploj, Sara
    Frati, Luigi
    Midulla, Cecilia
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2011, 12 (01): : 627 - 632
  • [2] Anthony AM, 2000, CLIN CHEM, V46, P1719
  • [3] BARBATI A, 1993, CLIN CHEM, V39, P1548
  • [4] A RADIOIMMUNOASSAY USING A MONOCLONAL-ANTIBODY TO MONITOR THE COURSE OF EPITHELIAL OVARIAN-CANCER
    BAST, RC
    KLUG, TL
    STJOHN, E
    JENISON, E
    NILOFF, JM
    LAZARUS, H
    BERKOWITZ, RS
    LEAVITT, T
    GRIFFITHS, CT
    PARKER, L
    ZURAWSKI, VR
    KNAPP, RC
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1983, 309 (15) : 883 - 887
  • [5] CA 125: The past and the future
    Bast, RC
    Xu, FJ
    Yu, YH
    Barnhill, S
    Zhang, Z
    Mills, GB
    [J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS, 1998, 13 (04) : 179 - 187
  • [6] WHAT DO WE KNOW ABOUT THE ORIGIN OF CA-125
    BISCHOF, P
    [J]. EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 1993, 49 (1-2): : 93 - 98
  • [7] A PROZONE PHENOMENON INTERFERES IN ISLET CELL ANTIBODY DETECTION - DIRECT COMPARISON OF 2 METHODS IN SUBJECTS AT RISK OF DIABETES AND IN INSULIN DEPENDENT DIABETICS AT ONSET
    COLMAN, PG
    DIMARIO, U
    RABIZADEH, A
    DOTTA, F
    ANASTASI, E
    EISENBARTH, GS
    [J]. JOURNAL OF AUTOIMMUNITY, 1988, 1 (02) : 109 - 117
  • [8] Tumor Markers in Clinical Practice: A Review Focusing on Common Solid Cancers
    Duffy, Michael J.
    [J]. MEDICAL PRINCIPLES AND PRACTICE, 2013, 22 (01) : 4 - 11
  • [9] CA125 in ovarian cancer: European Group on Tumor Markers guidelines for clinical use
    Duffy, MJ
    Bonfrer, JM
    Kulpa, J
    Rustin, GJS
    Soletormos, G
    Torre, GC
    Tuxen, MK
    Zwirner, M
    [J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2005, 15 (05) : 679 - 691
  • [10] Role of HE4, CA72.4, and CA125 in monitoring ovarian cancer
    Granato, Teresa
    Midulla, Cecilia
    Longo, Flavia
    Colaprisca, Barbara
    Frati, Luigi
    Anastasi, Emanuela
    [J]. TUMOR BIOLOGY, 2012, 33 (05) : 1335 - 1339