Restoration of chondrogenic properties of degenerative nucleus pulposus cells by repeated co-culture with adipose-derived stem cells

被引：4

作者：

Kim, Jin-Su ^{[1
,2
]}

Kwon, Daegi ^{[1
]}

Cha, Byung-Hyun ^{[1
]}

Moon, Bo Kyung ^{[1
]}

Jeong, Younghyen ^{[3
]}

Han, In-Bo ^{[4
]}

Park, Hansoo ^{[3
]}

Lee, Soo-Hong ^{[1
]}

机构：

[1] CHA Univ, Dept Biomed Sci, 335 Pangyo Ro, Seongnam Si 13488, Gyeonggi Do, South Korea

[2] TJC LIFE Co Ltd, R&D Ctr, 473-3 Bangbae Dong, Seoul 06698, South Korea

[3] Chung Ang Univ, Sch Integrat Engn, 84 Heukseok Ro, Seoul 06974, South Korea

[4] CHA Univ, Bundang Med Ctr, Dept Neurosurg, Seongnam Si 13496, Gyeonggi Do, South Korea

来源：

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | 2018年 / 60卷

基金：

新加坡国家研究基金会;

关键词：

Repeated co-culture; Chondrogenic differentiation; Degenerated; Nucleus pulposus cell; INTERVERTEBRAL DISC; STROMAL CELLS; TISSUE; DIFFERENTIATION; REGENERATION; CHONDROCYTES; MODULATION; SYSTEM; PROGRESSION; PHENOTYPE;

D O I：

10.1016/j.jiec.2017.11.004

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We used porous membranes for co-culturing adipose-derived stem cells (ASCs) with nucleus pulposus cells (NPCs) to investigate whether repeated co-cultures restored the chondrogenic properties of degenerative NPCs (dNPCs). Human dNPCs were co-cultured with ASCs for 1 week and co-cultured again with new ASCs for 1 week. The expression levels of chondrogenic genes and GAGs were significantly higher in second co-cultured dNPCs than those in the first co-cultured dNPCs and in dNPCs cultured alone, respectively. After six weeks of in vivo transplantation, compared to first co-cultured dNPCs, second co-cultured dNPCs showed a significantly higher cartilage tissue formation. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

引用

页码：185 / 191

页数：7

共 32 条

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