Effects of Actin Cytoskeleton Disruption on Electroporation In Vitro

被引：20

作者：

Kim, Hong Bae ^{[1
]}

Lee, Seho ^{[2
]}

Chung, Jong Hoon ^{[1
]}

Kim, Seong Nam ^{[3
]}

Sung, Chang Kyu ^{[4
]}

Baik, Ku Youn ^{[5
]}

机构：

[1] Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul 08826, South Korea

[2] Korea Univ, Dept Brain & Cognit Engn, Seoul 02841, South Korea

[3] Stand Co Ltd, Med Engn Res Ctr, Gyeonggi Do 15880, South Korea

[4] Seoul Natl Univ, Canc Res Inst, SNU SMG Boramae Med Ctr, Dept Radiol,Coll Med, Seoul 03080, South Korea

[5] Kwangwoon Univ, Dept Biol & Elect Phys, Seoul 01897, South Korea

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2020年 / 191卷 / 04期

关键词：

Electroporation; Membrane potential; Membrane stiffness; Cytoskeleton; Cytochalasin D; PULSED ELECTRIC-FIELD; CELL-LINES; MEMBRANE; CALCIUM; MODEL; SIZE;

D O I：

10.1007/s12010-020-03271-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of actin fibers in cellular responses to external electric pulses is not clear yet. In this study, we utilized the blocker of actin polymerization, cytochalasin D (cytoD), and investigated its effects on the electropore generation. Eight 100 mu s electric pulses of sub-kilovolt per centimeter voltage with 100 ms intervals were applied to adhered cells in vitro, and the membrane permeability was quantified using membrane-impermeable propidium iodide (PI) dye. With cytoD application, the transfer of PI dye decreased significantly in all the applied voltages. At the same time, the roughness of cells increased, the membrane stiffness decreased, and the transmembrane resting potential decreased. Our result supports that actin fibers have clear effects on electroporation through modulating membrane properties including transmembrane resting potential.

引用

页码：1545 / 1561

页数：17

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