High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

被引：177
作者：
Aires-de-Sousa, M
Boye, K
de Lencastre, H
Deplano, A
Enright, MC
Etienne, J
Friedrich, A
Harmsen, D
Holmes, A
Huijsdens, XW
Kearns, AM
Mellmann, A
Meugnier, H
Rasheed, JK
Spalburg, E
Strommenger, B
Struelens, MJ
Tenover, FC
Thomas, J
Vogel, U
Westh, H
Xu, J
Witte, W
机构：
[1] Univ Hosp Munster, Dept Periodontol, D-48149 Munster, Germany
[2] Univ Hosp Munster, Inst Hyg, D-48149 Munster, Germany
[3] Hop Edouard Herriot, Cent Microbiol Lab, Lyon, France
[4] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
[5] Univ Libre Bruxelles, Hop Erasme, Dept Microbiol, Brussels, Belgium
[6] Hvidovre Univ Hosp, Dept Clin Microbiol, Hvidovre, Denmark
[7] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal
[8] Hlth Protect Agcy, Ctr Infect, London, England
[9] Natl Inst Publ Hlth & Environm, Diagnost Lab Infect Dis, NL-3720 BA Bilthoven, Netherlands
[10] Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA USA
[11] Robert Koch Inst, Wernigerode, Germany
[12] Univ Hosp Wurzburg, Inst Hyg & Microbiol, Wurzburg, Germany
[13] Natl Inst Communicable Dis Prevent & Control, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 02期
关键词：
D O I：
10.1128/JCM.44.2.619-621.2006
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
引用
收藏
页码：619 / 621
页数：3
相关论文
共 17 条
  • [1] Inter-centre comparison of pulsed-field gel electrophoresis for the typing of methicillin-resistant Staphylococcus aureus
    Cookson, BD
    Aparicio, P
    Deplano, A
    Struelens, M
    Goering, R
    Marples, R
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 44 (03) : 179 - 184
  • [2] Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive-element PCR analysis
    Deplano, A
    Schuermans, A
    van Eldere, J
    Witte, W
    Meugnier, H
    Etienne, J
    Grundmann, H
    Jonas, D
    Noordhoek, GT
    Dijkstra, J
    van Belkum, A
    van Leeuwen, W
    Tassios, PT
    Legakis, NJ
    van der Zee, A
    Bergmans, A
    Blanc, DS
    Tenover, FC
    Cookson, BC
    O'Neil, G
    Struelens, MJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (10) : 3527 - 3533
  • [3] Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus
    Enright, MC
    Day, NPJ
    Davies, CE
    Peacock, SJ
    Spratt, BG
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) : 1008 - 1015
  • [4] The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA)
    Enright, MC
    Robinson, DA
    Randle, G
    Feil, EJ
    Grundmann, H
    Spratt, BG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (11) : 7687 - 7692
  • [5] Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism
    Frenay, HME
    Bunschoten, AE
    Schouls, LM
    vanLeeuwen, WJ
    VandenbrouckeGrauls, CMJE
    Verhoef, J
    Mooi, FR
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1996, 15 (01) : 60 - 64
  • [6] Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management
    Harmsen, D
    Claus, H
    Witte, W
    Rothgänger, J
    Claus, H
    Turnwald, D
    Vogel, U
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (12) : 5442 - 5448
  • [7] spa Typing method for discriminating among Staphylococcus aureus isolates:: Implications for use of a single marker to detect genetic micro- and macrovariation
    Koreen, L
    Ramaswamy, SV
    Graviss, EA
    Naidich, S
    Musser, JA
    Kreiswirth, BN
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (02) : 792 - 799
  • [8] MELLMANN A, IN PRESS PLOS MED
  • [9] Towards "Molecular esperanto" or the tower of babel?: (The need for harmonization of techniques for genotyping clinical isolates of Pseudomonas aeruginosa isolated from patients with cystic fibrosis)
    Moore, JE
    Goldsmith, CE
    Elborn, JS
    Murphy, PG
    Gilligan, PH
    Fanning, S
    Hogg, G
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (11) : 5347 - 5348
  • [10] Development of a Canadian standardized protocol for subtyping methicillin-resistant Staphylococcus aureus using pulsed-field gel electrophoresis
    Mulvey, MR
    Chui, L
    Ismail, J
    Louie, L
    Murphy, C
    Chang, N
    Alfa, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (10) : 3481 - 3485
12