High interlaboratory reproducibility of DNA sequence-based typing of bacteria in a multicenter study

被引：177
作者：
Aires-de-Sousa, M
Boye, K
de Lencastre, H
Deplano, A
Enright, MC
Etienne, J
Friedrich, A
Harmsen, D
Holmes, A
Huijsdens, XW
Kearns, AM
Mellmann, A
Meugnier, H
Rasheed, JK
Spalburg, E
Strommenger, B
Struelens, MJ
Tenover, FC
Thomas, J
Vogel, U
Westh, H
Xu, J
Witte, W
机构：
[1] Univ Hosp Munster, Dept Periodontol, D-48149 Munster, Germany
[2] Univ Hosp Munster, Inst Hyg, D-48149 Munster, Germany
[3] Hop Edouard Herriot, Cent Microbiol Lab, Lyon, France
[4] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England
[5] Univ Libre Bruxelles, Hop Erasme, Dept Microbiol, Brussels, Belgium
[6] Hvidovre Univ Hosp, Dept Clin Microbiol, Hvidovre, Denmark
[7] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal
[8] Hlth Protect Agcy, Ctr Infect, London, England
[9] Natl Inst Publ Hlth & Environm, Diagnost Lab Infect Dis, NL-3720 BA Bilthoven, Netherlands
[10] Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Atlanta, GA USA
[11] Robert Koch Inst, Wernigerode, Germany
[12] Univ Hosp Wurzburg, Inst Hyg & Microbiol, Wurzburg, Germany
[13] Natl Inst Communicable Dis Prevent & Control, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 02期
关键词：
D O I：
10.1128/JCM.44.2.619-621.2006
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
引用
收藏
页码：619 / 621
页数：3
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