Stepwise Assembly and Characterization of DNA Linked Two-Color Quantum Dot Clusters

被引:13
作者
Coopersmith, Kaitlin [1 ]
Han, Hyunjoo [1 ]
Maye, Mathew M. [1 ]
机构
[1] Syracuse Univ, Dept Chem, Syracuse, NY 13244 USA
基金
美国国家科学基金会;
关键词
RESONANCE ENERGY-TRANSFER; HIGH-PURITY SEPARATION; SEMICONDUCTOR NANOCRYSTALS; NANOPARTICLE DIMERS; AU NANOPARTICLE; PHOTOLUMINESCENCE; CENTRIFUGATION; NANOSTRUCTURES; ORGANIZATION; REAGENTS;
D O I
10.1021/acs.langmuir.5b01130
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The DNA-mediated self-assembly of multicolor quantum dot (QD) clusters via a stepwise approach is described. The CdSe/ZnS QDs were synthesized and functionalized with an amphiphilic copolymer, followed by ssDNA conjugation. At each functionalization step, the QDs were purified via gradient ultracentrifugation, which was found to remove excess polymer and QD aggregates, allowing for improved conjugation yields and assembly reactivity. The QDs were then assembled and disassembled in a stepwise manner at a ssDNA functionalized magnetic colloid, which provided a convenient way to remove unreacted QDs and ssDNA impurities. After assembly/disassembly, the clusters' optical characteristics were studied by fluorescence spectroscopy and the assembly morphology and stoichiometry was imaged via electron microscopy. The results indicate that a significant amount of QD-to-QD energy transfer occurred in the clusters, which was studied as a function of increasing acceptor-to-donor ratios, resulting in increased QD acceptor emission intensities compared to controls.
引用
收藏
页码:7463 / 7471
页数:9
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