Characterization of Genes Involved in D-Sorbitol Oxidation in Thermotolerant Gluconobacter frateurii

被引：9

作者：

Soemphol, Wichai ^{[1
]}

Saichana, Natsaran ^{[2
]}

Yakushi, Toshiharu ^{[2
]}

Adachi, Osao ^{[2
]}

Matsushita, Kazunobu ^{[2
]}

Toyama, Hirohide

机构：

[1] Khon Kaen Univ, Dept Sci & Technol, Nongkhai 43000, Thailand

[2] Yamaguchi Univ, Dept Biol Chem, Fac Agr, Yamaguchi 7538515, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 08期

基金：

日本学术振兴会;

关键词：

D-sorbitol dehydrogenase; glycerol dehydrogenase; xylitol dehydrogenase; AraC/XylE regulator family; major facilitator superfamily; XYLITOL DEHYDROGENASE; D-FRUCTOSE; MEMBRANE; CLONING; PURIFICATION; ACID; METABOLISM; CATABOLISM; PATHWAYS; STRAINS;

D O I：

10.1271/bbb.120227

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Further upstream of sldSLC, genes for FAD-dependent D-sorbitol dehydrogenase in Gluconobacter frateurii, three additional genes (sldR, xdhA, and perA) are found: for a transcriptional regulator, NAD(P)-dependent xylitol dehydrogenase, and a transporter protein, a member of major facilitator superfamily, respectively. xdhA and perA but not sldR were found to be in the same transcriptional unit. Disruption of sldR resulted in a dramatic decrease in sldSLC promoter activity, indicating that it is an activator for sldSLC expression. The recombinant protein of XdhA expressed in Escherichia coli showed NAD-dependent dehydrogenase activities with xylitol and D-sorbitol, but a mutant strain defective in this gene showed similar activities with both substrates as compared to the wild-type strain. Nonetheless, the growth of the xdhA mutant strain on D-sorbitol and xylitol was retarded, and so was that of a mutant strain defective in perA. These results indicate that xdhA and perA are involved in assimilation of D-sorbitol and xylitol.

引用

页码：1497 / 1505

页数：9