In vitro modeling for inherited neurological diseases using induced pluripotent stem cells: from 2D to organoid

被引:12
|
作者
Nam, Ki Hong [1 ]
Yi, Sang Ah [1 ]
Jang, Hyun Ji [1 ]
Han, Jeung-Whan [1 ]
Lee, Jaecheol [1 ,2 ,3 ]
机构
[1] Sungkyunkwan Univ, Sch Pharm, Suwon 16419, South Korea
[2] Sungkyunkwan Univ, SKKU BICS, Biomed Inst Convergence, Suwon 16419, South Korea
[3] Imnewrun Biosci Inc, Suwon 16419, South Korea
基金
新加坡国家研究基金会;
关键词
Stem cell; Neurogenesis; Brain organoid; Disease modeling; Neurodevelopmental disease; MILLER-DIEKER-SYNDROME; PRIMARY MICROCEPHALY MCPH; RETT-SYNDROME; CEREBRAL ORGANOIDS; DOWN-SYNDROME; CORTICAL DEVELOPMENT; HUMAN ES; DIRECTED DIFFERENTIATION; NEURAL PROGENITORS; BRAIN ORGANOIDS;
D O I
10.1007/s12272-020-01260-z
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Stem cells are characterized by self-renewal and by their ability to differentiate into cells of various organs. With massive progress in 2D and 3D cell culture techniques, in vitro generation of various types of such organoids from patient-derived stem cells is now possible. As in vitro differentiation protocols are usually made to resemble human developmental processes, organogenesis of patient-derived stem cells can provide key information regarding a range of developmental diseases. Human stem cell-based in vitro modeling as opposed to using animal models can particularly benefit the evaluation of neurological diseases because of significant differences in structure and developmental processes between the human and the animal brain. This review focuses on stem cell-based in vitro modeling of neurodevelopmental disorders, more specifically, the fundamentals and technical advancements in monolayer neuron and brain organoid cultures. Furthermore, we discuss the drawbacks of the conventional culture method and explore the advanced, cutting edge 3D organoid models for several neurodevelopmental diseases, including genetic diseases such as Down syndrome, Rett syndrome, and Miller-Dieker syndrome, as well as brain malformations like macrocephaly and microcephaly. Finally, we discuss the limitations of the current organoid techniques and some potential solutions that pave the way for accurate modeling of neurological disorders in a dish.
引用
收藏
页码:877 / 889
页数:13
相关论文
共 50 条
  • [41] Modeling Keratoconus Using Induced Pluripotent Stem Cells
    Joseph, Roy
    Srivastava, Om P.
    Pfister, Roswell R.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2016, 57 (08) : 3685 - 3697
  • [42] Induced pluripotent stem cells (iPSCs) and neurological disease modeling: progress and promises
    Marchetto, Maria C.
    Brennand, Kristen J.
    Boyer, Leah F.
    Gage, Fred H.
    HUMAN MOLECULAR GENETICS, 2011, 20 : R109 - R115
  • [43] In Vitro Modeling of Paraxial Mesodermal Progenitors Derived from Induced Pluripotent Stem Cells
    Sakurai, Hidetoshi
    Sakaguchi, Yasuko
    Shoji, Emi
    Nishino, Tokiko
    Maki, Izumi
    Sakai, Hiroshi
    Hanaoka, Kazunori
    Kakizuka, Akira
    Sehara-Fujisawa, Atsuko
    PLOS ONE, 2012, 7 (10):
  • [44] Studying neurological disorders using induced pluripotent stem cells and optogenetics
    Chin, Eunice W. M.
    Goh, Eyleen L. K.
    NEURAL REGENERATION RESEARCH, 2015, 10 (11) : 1720 - 1722
  • [45] Studying neurological disorders using induced pluripotent stem cells and optogenetics
    Eunice W.M.Chin
    Eyleen L.K.Goh
    NeuralRegenerationResearch, 2015, 10 (11) : 1720 - 1722
  • [46] Research on neurodegenerative diseases using induced pluripotent stem cells
    Imamura, Keiko
    Inoue, Haruhisa
    PSYCHOGERIATRICS, 2012, 12 (02) : 115 - 119
  • [47] Concise Review: Modeling Central Nervous System Diseases Using Induced Pluripotent Stem Cells
    Zeng, Xianmin
    Hunsberger, Joshua G.
    Simeonov, Anton
    Malik, Nasir
    Pei, Ying
    Rao, Mahendra
    STEM CELLS TRANSLATIONAL MEDICINE, 2014, 3 (12) : 1418 - 1428
  • [48] Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells
    Montel-Hagen, Amelie
    Seet, Christopher S.
    Li, Suwen
    Chick, Brent
    Zhu, Yuhua
    Chang, Patrick
    Tsai, Steven
    Sun, Victoria
    Lopez, Shawn
    Chen, Ho-Chung
    He, Chongbin
    Chin, Chee Jia
    Casero, David
    Crooks, Gay M.
    CELL STEM CELL, 2019, 24 (03) : 376 - +
  • [49] Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid system
    Sheng Cui
    Xianying Fang
    Hanbi Lee
    Yoo Jin Shin
    Eun-Sil Koh
    Sungjin Chung
    Hoon Suk Park
    Sun Woo Lim
    Kang In Lee
    Jae Young Lee
    Chul Woo Yang
    Byung Ha Chung
    Journal of Translational Medicine, 21
  • [50] Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid system
    Cui, Sheng
    Fang, Xianying
    Lee, Hanbi
    Shin, Yoo Jin
    Koh, Eun-Sil
    Chung, Sungjin
    Park, Hoon Suk
    Lim, Sun Woo
    Lee, Kang In
    Lee, Jae Young
    Yang, Chul Woo
    Chung, Byung Ha
    JOURNAL OF TRANSLATIONAL MEDICINE, 2023, 21 (01)