In vitro modeling for inherited neurological diseases using induced pluripotent stem cells: from 2D to organoid

被引:12
|
作者
Nam, Ki Hong [1 ]
Yi, Sang Ah [1 ]
Jang, Hyun Ji [1 ]
Han, Jeung-Whan [1 ]
Lee, Jaecheol [1 ,2 ,3 ]
机构
[1] Sungkyunkwan Univ, Sch Pharm, Suwon 16419, South Korea
[2] Sungkyunkwan Univ, SKKU BICS, Biomed Inst Convergence, Suwon 16419, South Korea
[3] Imnewrun Biosci Inc, Suwon 16419, South Korea
基金
新加坡国家研究基金会;
关键词
Stem cell; Neurogenesis; Brain organoid; Disease modeling; Neurodevelopmental disease; MILLER-DIEKER-SYNDROME; PRIMARY MICROCEPHALY MCPH; RETT-SYNDROME; CEREBRAL ORGANOIDS; DOWN-SYNDROME; CORTICAL DEVELOPMENT; HUMAN ES; DIRECTED DIFFERENTIATION; NEURAL PROGENITORS; BRAIN ORGANOIDS;
D O I
10.1007/s12272-020-01260-z
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Stem cells are characterized by self-renewal and by their ability to differentiate into cells of various organs. With massive progress in 2D and 3D cell culture techniques, in vitro generation of various types of such organoids from patient-derived stem cells is now possible. As in vitro differentiation protocols are usually made to resemble human developmental processes, organogenesis of patient-derived stem cells can provide key information regarding a range of developmental diseases. Human stem cell-based in vitro modeling as opposed to using animal models can particularly benefit the evaluation of neurological diseases because of significant differences in structure and developmental processes between the human and the animal brain. This review focuses on stem cell-based in vitro modeling of neurodevelopmental disorders, more specifically, the fundamentals and technical advancements in monolayer neuron and brain organoid cultures. Furthermore, we discuss the drawbacks of the conventional culture method and explore the advanced, cutting edge 3D organoid models for several neurodevelopmental diseases, including genetic diseases such as Down syndrome, Rett syndrome, and Miller-Dieker syndrome, as well as brain malformations like macrocephaly and microcephaly. Finally, we discuss the limitations of the current organoid techniques and some potential solutions that pave the way for accurate modeling of neurological disorders in a dish.
引用
收藏
页码:877 / 889
页数:13
相关论文
共 50 条
  • [31] Modeling microglial differentiation and function in vitro using induced pluripotent stem cells
    Ginhoux, F.
    GLIA, 2017, 65 : E44 - E44
  • [32] Harnessing induced pluripotent stem cells for the modeling and treatment of neurological disorders
    Bar-Nur, Ori
    FUTURE NEUROLOGY, 2012, 7 (05) : 511 - 514
  • [33] Modeling Polyglutamine Expansion Diseases with Induced Pluripotent Stem Cells
    Naphade, Swati
    Tshilenge, Kizito-Tshitoko
    Ellerby, Lisa M.
    NEUROTHERAPEUTICS, 2019, 16 (04) : 979 - 998
  • [34] Modeling blood diseases with human induced pluripotent stem cells
    Georgomanoli, Maria
    Papapetrou, Eirini P.
    DISEASE MODELS & MECHANISMS, 2019, 12 (06)
  • [35] Modeling Polyglutamine Expansion Diseases with Induced Pluripotent Stem Cells
    Swati Naphade
    Kizito-Tshitoko Tshilenge
    Lisa M. Ellerby
    Neurotherapeutics, 2019, 16 : 979 - 998
  • [36] Modeling Neuropsychiatric and Neurodegenerative Diseases with induced Pluripotent Stem Cells
    LaMarca, Elizabeth A.
    Powell, Samuel K.
    Akbarian, Schahram
    Brennand, Kristen J.
    FRONTIERS IN PEDIATRICS, 2018, 6
  • [37] Retinal Organoids from Induced Pluripotent Stem Cells of Patients with Inherited Retinal Diseases: A Systematic Review
    Lee, Yoo Jin
    Jo, Dong Hyun
    STEM CELL REVIEWS AND REPORTS, 2025, 21 (01) : 167 - 197
  • [38] Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines
    Koki Fujimori
    Toshiki Tezuka
    Hiroyuki Ishiura
    Jun Mitsui
    Koichiro Doi
    Jun Yoshimura
    Hirobumi Tada
    Takuya Matsumoto
    Miho Isoda
    Ryota Hashimoto
    Nubutaka Hattori
    Takuya Takahashi
    Shinichi Morishita
    Shoji Tsuji
    Wado Akamatsu
    Hideyuki Okano
    Molecular Brain, 9
  • [39] Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines
    Fujimori, Koki
    Tezuka, Toshiki
    Ishiura, Hiroyuki
    Mitsui, Jun
    Doi, Koichiro
    Yoshimura, Jun
    Tada, Hirobumi
    Matsumoto, Takuya
    Isoda, Miho
    Hashimoto, Ryota
    Hattori, Nubutaka
    Takahashi, Takuya
    Morishita, Shinichi
    Tsuji, Shoji
    Akamatsu, Wado
    Okano, Hideyuki
    MOLECULAR BRAIN, 2016, 9 : 1 - 14
  • [40] Vascular Organoid Generation from Human-Induced Pluripotent Stem Cells
    Xu, Cong
    He, Siyu
    Zhu, Yuefei
    Crasto, Gazelle
    Chen, Caroline
    Clay, Morgan L.
    Lao, Yeh-Hsing
    Leong, Kam W.
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2024, (214):