Heterogeneous time dependent static light scattering

被引：11

作者：

Schimanowski, R ^{[1
]}

Strelitzki, R ^{[1
]}

Mullin, DA ^{[1
]}

Reed, WF ^{[1
]}

机构：

[1] Tulane Univ, Dept Phys, New Orleans, LA 70118 USA

来源：

MACROMOLECULES | 1999年 / 32卷 / 21期

关键词：

D O I：

10.1021/ma9907690

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Measurements of light scattering from flowing heterogeneous solutions containing coexisting populations of colloids and polymers are presented. By appropriate recognition of scattering peaks produced by individual colloid particles and recovery of the baseline scattering due to the polymer population, the colloid particle number density can be monitored in real time while the polymer population is characterized absolutely according to weight-average molecular weight M-w and z-average mean square radius of gyration < S-2>(z). Either or both populations may also be evolving in time, so that the technique is termed "heterogeneous time dependent static light scattering" (HTDSLS). The essential notions of the technique are presented, and then the feasibility of the simultaneous measurements is first demonstrated by an equilibrium characterization of poly(vinylpyrrolidone) (PVP) "contaminated" by known concentrations of 2 mu m latex spheres. The time dependent capabilities are then demonstrated by monitoring the growth of Escherichia coli bacteria in a solution containing PVP. It is expected that HTDSLS will find many applications, such as in biotechnology reactors, biomedical assays, and polymer reactors where gels or crystallites are produced in addition to polymer chains.

引用

页码：7055 / 7063

页数：9

共 36 条

[1]

3Coulter Wallace H., 1956, P NATL ELECT C, V12, P1034

[2]

BADER H, 1923, REV SCI INSTRUM, V101, P19

[3] ABSOLUTE OPTICAL CROSS SECTIONS OF CELLS AND CHLOROPLASTS [J].