Over-expression of SOCS-3 Gene Promotes IL-10 Production by JEG-3 Trophoblast Cells

被引：20
作者：
Dong, Q. [1 ,2 ]
Fan, R. [1 ]
Zhao, S. [1 ]
Wang, Y. [1 ]
机构：
[1] Louisiana State Univ, Hlth Sci Ctr, Dept Obstet & Gynecol, Shreveport, LA 71130 USA
[2] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin 150086, Heilongjiang, Peoples R China
来源：
PLACENTA | 2009年 / 30卷 / 01期
关键词：
SOCS-3; IL-6; IL-10; Trophoblasts; INTERLEUKIN-10; PROLIFERATION; DEFICIENCY; PREVENTS; PROTEINS; ABORTION; WOMEN; GP130; TH1;
D O I：
10.1016/j.placenta.2008.10.008
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Suppressor of cytokine signaling-3 (SOCS-3) plays an important role in negative regulation of inflammatory response. Evidence has shown that SOCS-3 and IL-10 expressions were significantly reduced in placental trophoblasts from preeclampsia. IL-10 is an anti-inflammatory cytokine. In this study, we sought to determine if enhance SOCS-3 expression could affect IL-10 production in placental trophoblasts. Placental JEG-3 cells were used. Over-expression of SOCS-3 was generated by transfection of JEG-3 cells with a green fluorescent protein (GFP) tagged SOCS-3 gene, SOCS-3/ZsGreen1, by siPORT lipid transfection. Cells transfected with ZsGreen1 vector only was used as control. Our results showed that IL-6 production was reduced in cells over-expressed with SOCS-3. Moreover, SOCS-3 transfected cells produced more IL-10 when stimulated with IL-6. The increased IL-10 production by JEC-3 cells was in a dose-dependent manner, p < 0.05. Our data suggested that enhanced SOCS-3 gene expression could promote IL-10 production by placental trophoblast cells, suggesting that SOCS-3 may play an important role in regulation of cytokine induced anti-inflammatory response in placental trophoblasts. (C) 2008 Elsevier Ltd. All rights reserved.
引用
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页码：11 / 14
页数：4
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