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Method for detection of single-base mismatches using bimolecular beacons
被引:61
作者
:
Frutos, AG
论文数:
0
引用数:
0
h-index:
0
机构:
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Frutos, AG
[
1
]
Pal, S
论文数:
0
引用数:
0
h-index:
0
机构:
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Pal, S
[
1
]
Quesada, M
论文数:
0
引用数:
0
h-index:
0
机构:
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Quesada, M
[
1
]
Lahiri, J
论文数:
0
引用数:
0
h-index:
0
机构:
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
Lahiri, J
[
1
]
机构
:
[1]
Corning Inc, Div Sci & Technol, Corning, NY 14831 USA
来源
:
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
|
2002年
/ 124卷
/ 11期
关键词
:
D O I
:
10.1021/ja012374d
中图分类号
:
O6 [化学];
学科分类号
:
0703 ;
摘要
:
This paper describes a method for the detection of single-base mismatches using DNA microarrays in a format that does not require labeling of the sample ("target") DNA. The method is based on disrupting fluorescence energy transfer (FRET) between a fluorophore attached to an immobilized DNA strand ("probe") and a quencher-containing sequence that is complementary except for an artificial mismatch (e.g. 5-nitroindole, 3-nitropyrole, or abasic site) at the site of interrogation. As the displacement of the FRET acceptor and hybridization of the unlabeled probe are bimolecular, the term "bimolecular beacons" is used to describe this approach. The analysis of a mismatch was based on differences in the amount of disruption in FRET upon hybridization of perfectly matched DNA targets and those containing single-base mismatches. Using this method and an oligonucleotide model system, A/C single-base mismatches were successfully discriminated at levels greater than that observed using surface-immobilized molecular beacons. The amount of discrimination was dependent on the identity of the artificial mismatch; greater discrimination was observed with 5-nitroindole (a "universal" base) than with an abasic site. G/T mismatches, considered to be particularly difficult to detect, were also successfully discriminated when quencher sequences containing 5-nitroindole were used. Copyright © 2002 American Chemical Society.
引用
收藏
页码:2396 / 2397
页数:2
相关论文
共 22 条
[1]
BASE-BASE MISMATCHES - THERMODYNAMICS OF DOUBLE HELIX FORMATION FOR DCA3XA3G + DCT3YT3G (X, Y = A,C,G,T)
ABOULELA, F
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
ABOULELA, F
KOH, D
论文数:
0
引用数:
0
h-index:
0
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UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
KOH, D
TINOCO, I
论文数:
0
引用数:
0
h-index:
0
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UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
TINOCO, I
MARTIN, FH
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
MARTIN, FH
[J].
NUCLEIC ACIDS RESEARCH,
1985,
13
(13)
: 4811
-
4824
[2]
[Anonymous], NONISOTOPIC DNA PROB
[3]
Comparison of the base pairing properties of a series of nitroazole nucleobase analogs in the oligodeoxyribonucleotide sequence 5'-d(CGCXAATTYGCG)-3'
Bergstrom, DE
论文数:
0
引用数:
0
h-index:
0
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WALTHER CANC INST,INDIANAPOLIS,IN 46208
WALTHER CANC INST,INDIANAPOLIS,IN 46208
Bergstrom, DE
Zhang, PM
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0
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WALTHER CANC INST,INDIANAPOLIS,IN 46208
Zhang, PM
Johnson, WT
论文数:
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0
h-index:
0
机构:
WALTHER CANC INST,INDIANAPOLIS,IN 46208
WALTHER CANC INST,INDIANAPOLIS,IN 46208
Johnson, WT
[J].
NUCLEIC ACIDS RESEARCH,
1997,
25
(10)
: 1935
-
1942
[4]
Thermodynamic basis of the enhanced specificity of structured DNA probes
Bonnet, G
论文数:
0
引用数:
0
h-index:
0
机构:
Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Bonnet, G
Tyagi, S
论文数:
0
引用数:
0
h-index:
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Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Tyagi, S
Libchaber, A
论文数:
0
引用数:
0
h-index:
0
机构:
Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Libchaber, A
Kramer, FR
论文数:
0
引用数:
0
h-index:
0
机构:
Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Kramer, FR
[J].
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1999,
96
(11)
: 6171
-
6176
[5]
The essence of SNPs
Brookes, AJ
论文数:
0
引用数:
0
h-index:
0
机构:
Uppsala Univ, Ctr Biomed, Dept Genet & Pathol, S-75123 Uppsala, Sweden
Uppsala Univ, Ctr Biomed, Dept Genet & Pathol, S-75123 Uppsala, Sweden
Brookes, AJ
[J].
GENE,
1999,
234
(02)
: 177
-
186
[6]
Exploring the new world of the genome with DNA microarrays
Brown, PO
论文数:
0
引用数:
0
h-index:
0
机构:
Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
Brown, PO
Botstein, D
论文数:
0
引用数:
0
h-index:
0
机构:
Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
Botstein, D
[J].
NATURE GENETICS,
1999,
21
(Suppl 1)
: 33
-
37
[7]
Designing a novel molecular beacon for surface-immobilized DNA hybridization studies
Fang, XH
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Fang, XH
Liu, XJ
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Liu, XJ
Schuster, S
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Schuster, S
Tan, WH
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Tan, WH
[J].
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
1999,
121
(12)
: 2921
-
2922
[8]
Energy-transfer fluorescent reagents for DNA analyses
Glazer, AN
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0
引用数:
0
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0
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UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
Glazer, AN
Mathies, RA
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
Mathies, RA
[J].
CURRENT OPINION IN BIOTECHNOLOGY,
1997,
8
(01)
: 94
-
102
[9]
Single-nucleotide polymorphism analysis by MALDI-TOF mass spectrometry
Griffin, TJ
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA
Griffin, TJ
Smith, LM
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA
Smith, LM
[J].
TRENDS IN BIOTECHNOLOGY,
2000,
18
(02)
: 77
-
84
[10]
Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization
Guo, Z
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
Guo, Z
Liu, QH
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
Liu, QH
Smith, LM
论文数:
0
引用数:
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h-index:
0
机构:
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
Smith, LM
[J].
NATURE BIOTECHNOLOGY,
1997,
15
(04)
: 331
-
335
←
1
2
3
→
共 22 条
[1]
BASE-BASE MISMATCHES - THERMODYNAMICS OF DOUBLE HELIX FORMATION FOR DCA3XA3G + DCT3YT3G (X, Y = A,C,G,T)
ABOULELA, F
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
ABOULELA, F
KOH, D
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
KOH, D
TINOCO, I
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
TINOCO, I
MARTIN, FH
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY,CHEM BIODYNAM LAB,BERKELEY,CA 94720
MARTIN, FH
[J].
NUCLEIC ACIDS RESEARCH,
1985,
13
(13)
: 4811
-
4824
[2]
[Anonymous], NONISOTOPIC DNA PROB
[3]
Comparison of the base pairing properties of a series of nitroazole nucleobase analogs in the oligodeoxyribonucleotide sequence 5'-d(CGCXAATTYGCG)-3'
Bergstrom, DE
论文数:
0
引用数:
0
h-index:
0
机构:
WALTHER CANC INST,INDIANAPOLIS,IN 46208
WALTHER CANC INST,INDIANAPOLIS,IN 46208
Bergstrom, DE
Zhang, PM
论文数:
0
引用数:
0
h-index:
0
机构:
WALTHER CANC INST,INDIANAPOLIS,IN 46208
WALTHER CANC INST,INDIANAPOLIS,IN 46208
Zhang, PM
Johnson, WT
论文数:
0
引用数:
0
h-index:
0
机构:
WALTHER CANC INST,INDIANAPOLIS,IN 46208
WALTHER CANC INST,INDIANAPOLIS,IN 46208
Johnson, WT
[J].
NUCLEIC ACIDS RESEARCH,
1997,
25
(10)
: 1935
-
1942
[4]
Thermodynamic basis of the enhanced specificity of structured DNA probes
Bonnet, G
论文数:
0
引用数:
0
h-index:
0
机构:
Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Bonnet, G
Tyagi, S
论文数:
0
引用数:
0
h-index:
0
机构:
Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Tyagi, S
Libchaber, A
论文数:
0
引用数:
0
h-index:
0
机构:
Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Libchaber, A
Kramer, FR
论文数:
0
引用数:
0
h-index:
0
机构:
Publ Hlth Res Inst, Dept Mol Genet, New York, NY 10016 USA
Kramer, FR
[J].
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1999,
96
(11)
: 6171
-
6176
[5]
The essence of SNPs
Brookes, AJ
论文数:
0
引用数:
0
h-index:
0
机构:
Uppsala Univ, Ctr Biomed, Dept Genet & Pathol, S-75123 Uppsala, Sweden
Uppsala Univ, Ctr Biomed, Dept Genet & Pathol, S-75123 Uppsala, Sweden
Brookes, AJ
[J].
GENE,
1999,
234
(02)
: 177
-
186
[6]
Exploring the new world of the genome with DNA microarrays
Brown, PO
论文数:
0
引用数:
0
h-index:
0
机构:
Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
Brown, PO
Botstein, D
论文数:
0
引用数:
0
h-index:
0
机构:
Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
Botstein, D
[J].
NATURE GENETICS,
1999,
21
(Suppl 1)
: 33
-
37
[7]
Designing a novel molecular beacon for surface-immobilized DNA hybridization studies
Fang, XH
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Fang, XH
Liu, XJ
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Liu, XJ
Schuster, S
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Schuster, S
Tan, WH
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Univ Florida, Dept Chem, Gainesville, FL 32601 USA
Tan, WH
[J].
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,
1999,
121
(12)
: 2921
-
2922
[8]
Energy-transfer fluorescent reagents for DNA analyses
Glazer, AN
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
Glazer, AN
Mathies, RA
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
UNIV CALIF BERKELEY, DEPT CHEM, BERKELEY, CA 94720 USA
Mathies, RA
[J].
CURRENT OPINION IN BIOTECHNOLOGY,
1997,
8
(01)
: 94
-
102
[9]
Single-nucleotide polymorphism analysis by MALDI-TOF mass spectrometry
Griffin, TJ
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA
Griffin, TJ
Smith, LM
论文数:
0
引用数:
0
h-index:
0
机构:
Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA
Smith, LM
[J].
TRENDS IN BIOTECHNOLOGY,
2000,
18
(02)
: 77
-
84
[10]
Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization
Guo, Z
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
Guo, Z
Liu, QH
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
Liu, QH
Smith, LM
论文数:
0
引用数:
0
h-index:
0
机构:
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
UNIV WISCONSIN,DEPT CHEM,MADISON,WI 53706
Smith, LM
[J].
NATURE BIOTECHNOLOGY,
1997,
15
(04)
: 331
-
335
←
1
2
3
→