Raman and fluorescence microscopy to study the internalization and dissolution of photosensitizer nanoparticles into living cells

被引:0
作者
Scalfi-Happ, Claudia [1 ]
Steiner, Rudolf [1 ]
Wittig, Rainer
Graefe, Susanna [2 ]
Ryabova, Anastasia [3 ,4 ]
Loschenov, Victor [3 ,4 ]
机构
[1] Inst Laser Technol Med & Metrol ILM, D-89081 Ulm, Germany
[2] Biolitec Res GmbH, D-07745 Jena, Germany
[3] BIOSPEC, Moscow 119991, Russia
[4] Russian Acad Sci, AM Prokhorov Gen Phys Inst, Moscow, Russia
来源
CLINICAL AND BIOMEDICAL SPECTROSCOPY AND IMAGING IV | 2015年 / 9537卷
关键词
Raman microscopy; confocal microscopy; photodynamic therapy;
D O I
10.1117/12.2183726
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In this present study we applied Raman and fluorescence microscopy to investigate the internalisation, cellular distribution and effects on cell metabolism of photosensitizer nanoparticles for photodynamic therapy in fibroblasts and macrophages.
引用
收藏
页数:7
相关论文
共 8 条
[1]   Fluorescence investigation of the detachment of aluminum phthalocyanine molecules from aluminum phthalocyanine nanoparticles in monocytes/macrophages and skin cells and their localization in monocytes/macrophages [J].
Breymayer, Jasmin ;
Rueck, Angelika ;
Ryabova, Anastasiya V. ;
Loschenov, Victor B. ;
Steiner, Rudolf W. .
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2014, 11 (03) :380-390
[2]  
Brodin N.P., 2014, TECHNOL CANCER RES T, DOI [10.1177/153303461455619., DOI 10.1177/153303461455619]
[3]   Foslip®-based photodynamic therapy as a means to improve wound healing [J].
Garrier, Julie ;
Bezdetnaya, Lina ;
Barlier, Catherine ;
Graefe, Susanna ;
Guillemin, Francois ;
D'Hallewin, Marie-Ange .
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2011, 8 (04) :321-327
[4]   The many facets of Raman spectroscopy for biomedical analysis [J].
Krafft, Christoph ;
Popp, Jurgen .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2015, 407 (03) :699-717
[5]   Raman and CARS microspectroscopy of cells and tissues [J].
Krafft, Christoph ;
Dietzek, Benjamin ;
Popp, Juergen .
ANALYST, 2009, 134 (06) :1046-1057
[6]  
Scalfi-Happ C, 2007, PROC SPIE, V6630, DOI 10.1117/12.729455
[7]   Label-Free Detection of Tumor Markers in a Colon Carcinoma Tumor Progression Model by Confocal Raman Microspectroscopy [J].
Scalfi-Happ, Claudia ;
Rueck, Angelika ;
Udart, Martin ;
Hauser, Carmen ;
Duerr, Christine ;
Kriebel, Martin .
CLINICAL AND BIOMEDICAL SPECTROSCOPY AND IMAGING III, 2013, 8798
[8]   mTHPC - A drug on its way from second to third generation photosensitizer? [J].
Senge, Mathias O. .
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2012, 9 (02) :170-179