Differentiation of human endometrial stem cells into endothelial-like cells on gelatin/chitosan/bioglass nanofibrous scaffolds

被引：37
作者：
Shamosi, Atefeh [1 ]
Mehrabani, Davood [2 ,3 ]
Azami, Mahmoud [1 ]
Ebrahimi-Barough, Somayeh [1 ]
Siavashi, Vahid [4 ]
Ghanbari, Hossein [5 ]
Sharifi, Esmaeel [1 ]
Roozafzoon, Reza [1 ]
Ai, Jafar [1 ,6 ]
机构：
[1] Univ Tehran Med Sci, Sch Adv Technol Med, Dept Tissue Engn & Appl Cell Sci, Tehran, Iran
[2] Shiraz Univ Med Sci, Stem Cell & Transgen Technol Res Ctr, Shiraz, Iran
[3] Univ Manitoba, Dept Regenerat Med, Winnipeg, MB, Canada
[4] Univ Tehran, Dept Clin Pathol, Fac Vet Med, Tehran, Iran
[5] Univ Tehran Med Sci, Sch Adv Technol Med, Dept Med Nanotechnol, Tehran, Iran
[6] Univ Tehran Med Sci, Imam Khomeini Hosp, Brain & Spinal Injury Res Ctr, Tehran, Iran
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2017年 / 45卷 / 01期
基金：
美国国家科学基金会;
关键词：
Endothelial cells; gelatin/chitosan/bioglass; human endometrial stem cells; nanofiber scaffold; BIOACTIVE GLASS; IN-VITRO; GROWTH-FACTORS; STROMAL CELLS; GELATIN; ANGIOGENESIS; CHITOSAN; EXPRESSION; VEGF; VASCULARIZATION;
D O I：
10.3109/21691401.2016.1138493
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The capacity of gelatin/chitosan/bioactive glass nanopowders (GEL/CS/BGNPs) scaffolds was investigated for increasing human endometrial stem cells (hEnSCs) differentiation into the endothelial cells in the presence of angiogenic factors. GEL/CS nanofibrous scaffold with different contents of BGNPs were fabricated and assessed. Expression of endothelial markers (CD31, vascular endothelial cadherin (VE-cadherin), and KDR) in differentiated cells was evaluated. Results showed the diameter of nanofiber increases with decreasing the BG content in GEL/CS scaffolds. Moreover, in vitro study indicated that the GEL/CS/BGNPs scaffold with 1.5% BGNPs content provided a suitable three-dimensional structure for endothelial cells differentiation. Thus, the GEL/CS/BGNPs scaffold can be recommended for blood vessels repair.
引用
收藏
页码：163 / 173
页数：11
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