Internal epitope tagging informed by relative lack of sequence conservation

被引：15

作者：

Burg, Leonard ^{[1
]}

Zhang, Karen ^{[1
]}

Bonawitz, Tristan ^{[1
]}

Grajevskaja, Viktorija ^{[1
,2
]}

Bellipanni, Gianfranco ^{[1
]}

Waring, Richard ^{[1
]}

Balciunas, Darius ^{[1
]}

机构：

[1] Temple Univ, Coll Sci & Technol, Dept Biol, Philadelphia, PA 19122 USA

[2] Vilnius Univ, Dept Zool, Fac Nat Sci, Vilnius, Lithuania

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

ZEBRAFISH GENOME; SPECIFICATION; TRANSPOSON; NETWORK; TBX18;

D O I：

10.1038/srep36986

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.

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页数：8

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