Establishment and characterization of a novel osteosarcoma cell line: CHOS

被引：12

作者：

Liu, Yunlu ^{[1
]}

Feng, Xiaobo ^{[1
]}

Zhang, Yukun ^{[1
]}

Jiang, Hongyan ^{[2
]}

Cai, Xianyi ^{[1
]}

Yan, Xinxin ^{[1
]}

Huang, Zengfa ^{[1
]}

Mo, Fengbo ^{[1
]}

Yang, Wen ^{[3
]}

Yang, Cao ^{[1
]}

Yang, Shuhua ^{[1
]}

Liu, Xianzhe ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Orthopaed, 1277 JieFang Ave, Wuhan 430022, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Otorhinolaryngol, 1277 JieFang Ave, Wuhan 430022, Peoples R China

[3] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Anesthesiol, 1277 JieFang Ave, Wuhan 430022, Peoples R China

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2016年 / 34卷 / 12期

基金：

中国国家自然科学基金;

关键词：

chondroblastic osteosarcoma; metastasis; elderly; cell line; Y-CHROMOSOME; PATIENTS OLDER; AGE; CHEMOTHERAPY; ROPIVACAINE; BUPIVACAINE; SEQUENCES; PATTERNS; PATIENT;

D O I：

10.1002/jor.23245

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Osteosarcoma has a well-recognized bimodal distribution, with the first peak in adolescence and another in the elderly age-group. The elderly patients have different clinical features and a poorer prognosis as compared to adolescents. To better understand the biological features of osteosarcoma in the elderly population, we established a new human osteosarcoma cell line from a 58-year-old man with primary chondroblastic osteosarcoma. After 6 months of continuous culture in vitro for over 50 passages, an immortalized cell line CHOS was established. The cell line was well-characterized by cytogenetic, biomarker, functional, and histological analyses. The CHOS cells exhibited a spindle-shaped morphology and a doubling time of 36h. Cytogenetic analysis of CHOS cells revealed the loss of chromosome Y and the gain of chromosome 12. Quantitative real-time polymerase chain reaction (RT-PCR), Western blotting and/or immunofluorescence revealed the expression of chondroblastic, mesenchymal and tumor metastasis markers in the CHOS cells. Compared with the osteosarcoma cell line, the CHOS cells were found to be more sensitive to cisplatin and doxorubicin, but were resistant to methotrexate. The cell line was highly tumorigenic and maintained the histological characteristics and invasive nature of the original tumor. Furthermore, on immunohistochemical analysis, the xenografts and metastases were found to co-express collagen II, aggrecan, vimentin and S100A4 that resembled the original tumor cells. Our results indicate, the potential of CHOS cell line to serve as a useful tool for further studies on the molecular biology of osteosarcoma, especially in the elderly patients. (c) 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:2116-2125, 2016.

引用

页码：2116 / 2125

页数：10

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